摘要
目的分析具核梭杆菌(Fn)在结直肠癌(CRC)患者癌组织与癌旁正常组织的定植情况,及其定植丰度在结直肠癌中的诊断价值。方法选择2016年1月至2016年11月于青岛市第八人民医院普外科行结直肠癌根治术且术后病理结果为腺癌的129例患者为研究对象,应用RT-qPCR技术检测其癌组织与癌旁正常组织中Fn定植情况并进行比较,并分析不同临床分期CRC患者中Fn定植率的差异,Fn阳性率与CEA和(或)CA19-9阳性率的差异,并应用ROC分析Fn定植丰度在CRC中的诊断价值。结果129例患者中84例(65.1%)癌组织标本检测到Fn定植,73例(56.5%)癌旁正常组织标本检测到Fn定植,两者定植率比较,差异无统计学意义(P>0.05);癌组织Fn定植丰度均值高于癌旁正常组织,差异有统计学意义(P<0.05)。不同TNM分期的CRC患者Fn阳性率差异无统计学意义(P>0.05);CRC患者中Fn阳性率与CEA和(或)CA19-9阳性率比较差异无统计学意义(P>0.05)。ROC分析结果显示Fn定植丰度诊断CRC的界值为7.335,AUC为0.911,此时敏感度为88.1%,特异度为84.5%,诊断符合率为86.3%。结论初步研究显示CRC患者癌组织和癌旁正常组织中Fn定植率没有明显差异,但在癌组织中定植丰度更高,这可能提示Fn在CRC的诊断等临床研究中可发挥一定的作用,但其应用价值有待更深入的研究探索。
Objectives To investigate the colorization of Fusobacterium nucleatum (Fn) in colorectal cancer tissue and paracan cerous normal tissue, and the diagnostic value of its abundance for colorectal cancer. Methods One hundred and twenty-nine colorectal cancer patients who received surgery between January 2016 and November 2016 at the Department of General Surgery, Qingdao Eighth Hospital were recruited. The colorization of Fn in colorectal cancer and paracancerous normal tissue were detect ed by RT-qPCR. The difference of Fn colorization rate in different stages of cancer and the difference between Fn positive rate and CEA and/or CA19-9 positive rate were analyzed. The ROC (Receiver Operating Curve) was performed to explore the diagnos tic value of its abundance for colorectal cancer. Results Eighty-four of the 129 patients had colorization of Fn, and the coloriza tion rate was 65.1% at colorectal cancer tissue. Seventy-three patients had colorization of Fn, and the colorization rate was 56.5% at paracancerous norrmal tissue. There was no significant difference in the colorization rate between colorectal cancer and paracancerous normal tissue ( P > 0.05). Average colorization abundance was significantly higher at colorectal cancer tissue than at paracancerous normal tissue ( P < 0.05). There was no significant difference in the positive rate of Fn in colorectal cancer pa tients with different TNM stages ( P > 0.05). The positive rate of Fn did not differ from that of the CEA and/or CA19-9 ( P > 0.05). The ROC data showed that the abundance cut-off value was 7.335, and the AUC , the sensitivity, the specificity, and the coincidence rate was 0.911, 88.1%, 84.5% and 86.3%, respectively. Conclusion The abundance of Fn was higher in colorectal cancer tissue than in paracancerous normal tissue, and it had a high diagnostic value for colorectal cancer. The colorization rate was equivalent in colorectal cancer tissue and paracancerous normal tissue. The application of Fn in the diagnosis and treatment of colorectal cancer re
作者
梁国刚
王群先
韩方海
Liang Guogang;Wang Qunxian;Han Fanghai(Department of General Surgery, Qingdao Eighth People’s Hospital, Qingdao 266100, Shandong, China;Department of Gastrointestinal Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510030, Guang dong, China)
出处
《结直肠肛门外科》
2019年第5期528-532,共5页
Journal of Colorectal & Anal Surgery
关键词
结直肠癌
具核梭杆菌
定植
诊断
colorectal cancer
Fusobacterium nucleatum
colorization
diagnosis
