摘要
目的应用兔动脉粥样硬化模型,联合正电子发射断层显像(PET)和CT血管造影检查,比较18F-氟化钠(18F-NaF)和18F-氟脱氧葡萄糖(18F-FDG)对动脉粥样硬化斑块微钙化的检测价值。方法选择雄性新西兰大白兔18只,采用球囊拉伤的方法制作动脉粥样硬化模型,术前2周及术后高脂饲料喂养。手术过程由于球囊拉伤血管破裂或术后感染死亡4只,共14只在第16周末接受PET/CT显像、病理及血液采集,其中2只未发现明显粥样硬化斑块及微钙化形成。取病变部位和邻近部位做钙化染色及免疫组织化学染色,比较2种显像最大标准化摄取值(SUVmax),分析钙化和炎症与PET显像的关系。结果病变部位与对照部位18F-FDG的SUVmax比较,差异无统计学意义(P>0.05)。与对照部位比较,病变部位18F-NaF的SUVmax明显升高(P<0.05)。病变部位18F-NaF的SUVmax与Von kossa及茜素红阳性面积百分比呈正相关(r=0.865,P<0.01;r=0.880,P<0.01),病变部位18F-FDG的SUVmax与Von kossa及茜素红不相关(r=-0.272,P>0.05;r=-0.085,P>0.05)。18F-NaF的SUVmax与病变部位CD68阳性面积百分比相关(r=0.714,P<0.01),但18F-FDG的SUVmax与病变部位CD68阳性面积百分比不相关(r=-0.405,P>0.05)。结论 18F-NaF较18F-FDG能够更准确地用于检测微钙化,并进一步发现易损斑块,可用于冠心病的诊断和预防。
Objective To compare the values of 18F-NaF and 18F-FDG in detecting microcalcification of atherosclerotic plaques using rabbit atherosclerosis model combined with PET and CTA.Methods Eighteen healthy male New Zealand rabbits were included in this study.An atherosclerosis model of rabbits was established by balloon strain.The animals were fed with high-fat diets for 2 weeks before and after operation.Four rabbits died of vascular rupture due to perioperative balloon strain or postoperative infection.Fourteen rabbits received PET/CT imaging by the end of week 16 with no atherosclerotic plaque and microcalcification detected in 2.The SUVmax of18F-FDG was compared and the relationship of calcification and inflammation with PET inmage was analyzed with pathological and immunohistochemical staining.Results No significant difference was detected in SUVmax of 18F-FDG between the lesion and control sites(P>0.05).The SUVmax of 18F-NaF was significantly higher at lesion site than at control site(P<0.05).The SUVmax of 18F-NaF at lesion site was positively related with Von kossa and alizarin red positive area percentage(r=0.865,P<0.01;r=0.880,P<0.01)while the SUVmax of 18F-FDG was not related with Von kossa and alizarin red negative area percentage(r=-0.272,P >0.05;r=-0.085,P>0.05).The SUVmax of 18F-NaF at lesion site was positively related with the percentage of CD68 positive area(r=0.714,P<0.01)while the SUVmax of 18F-FDG was not related with the percentage of CD68 positive area(r=-0.405,P>0.05).Conclusion The accuracy of 18F-NaF is higher than that of 18F-FDG in detecting microcalcification.Vulnerable plaques detected by 18F-NaF can be used as a marker for the diagnosis and prevention of CHD.
作者
陈雪
赵全明
张学慧
聂毛晓
闫云峰
梁喆
赵欣
冯婷婷
Chen Xue;Zhao Quanming;Zhang Xuehui;Nie Maoxiao;Yan Yunfeng;Liang Zhe;Zhao Xin;Feng Tingting(Department of Cardiology,Affiliated Beijing Anzhen Hospital of Capital Medical University Beijing 100029 ,China)
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2019年第10期1031-1035,共5页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金(81870368,81370437)
