摘要
建立LC-MS^n法对福白菊化学成分进行定性研究,鉴定了22个化学成分,包括13个黄酮类成分和9个酚酸类成分;采用HPLC-UV法,同一色谱条件下测定福白菊中5种化学成分含量,包括:绿原酸(1)、木犀草素-7-O-β-D-葡萄糖苷(2)、木犀草素-7-O-β-D-葡萄糖醛酸苷(3)、3,5-二咖啡酰基奎宁酸(4)、芹菜素-7-O-β-D-葡萄糖苷(5),建立5种化学成分的回归方程,线性关系均良好(r>0.999 9),加样回收率为98.72%~103.16%,采用此方法成功检测了10个福白菊样品中上述5种成分的含量。本文建立分析方法简便可靠,可定性及定量分析福白菊中的主要化学成分,适用于福白菊药材的质量分析。
A qualitative analytical method of liquid chromatography coupled with mass spectrometry(LC-MS^n) was developed for the identification of main constituents in Chrysanthemum morifolium ‘Fubaiju’.High-performance liquid chromatography(HPLC) was developed for the quantification of five active components,including chlorogenic acid(1),luteolin-7-O-β-D-glucopyranoside(2),luteolin-7-O-β-D-glucopyranuronide(3),3,5-Di-caffeoylquinic acid(4),and apigenin-7-O-β-D-glucopyranoside(5).A total of 22 compounds,including 13 flavonoids and 9 phenolic acids,were identified based on their retention behaviors,UV profiles and MS fragment information.Furthermore,a validation method with good linearity(r>0.999 9),precision,stability,repeatability and recovery was successfully applied for simultaneous determination of five major components in 10 batches of C.morifolium ‘Fubaiju’ by HPLC-UV method.The established method was proved to be a validation strategy for the quality evaluation of C.morifolium ‘Fubaiju’.
作者
李君彦
范氏英
孙中宣
程雪翔
叶文才
汪豪
LI Junyan;PHAM Thi Anh;SUN Zhongxuan;CHEN Xuexiang;YE Wencai;WANG Hao(Department of Pharmaceutics for TCMs,China Pharmaceutical University,Nanjing 210009;Hubei Fenghuang Baiyunshan Pharmaceutical Co.,Ltd.,Macheng 438300;Institute of Traditional Chinese Medicine & Natural Products,College of Pharmacy,Jinan University,Guangzhou 510632,China)
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2019年第5期565-571,共7页
Journal of China Pharmaceutical University
基金
珠江人才计划本土创新团队资助项目(No.2017BT01Y036)
国家重点研发计划资助项目(No.2017YFC1703802)~~
关键词
福白菊
LC-MS^n
HPLC-UV
定性分析
含量测定
Chrysanthemum morifolium ’Fubaiju’
LC-MS^n
HPLC-UV
qualitative analysis
content determination
