摘要
通过向阴极室投加接种污泥构建阴极功能型的微生物燃料电池(MFC),并用其强化降解对硝基苯酚(PNP),考察了MFC运行过程中电极液pH、电导率和温度等环境因子的变化,使用SPSS13.0统计分析软件考察了各环境因子与MFC输出电压的相关关系,并对阴极生物膜样品采用高通量测序分析其菌群结构。结果表明,MFC输出电压与阳极室、阴极室pH均呈极显著相关关系,电极液pH为6时MFC对PNP的降解性能较优,PNP降解率为100.0%,还原降解中间产物对氨基苯酚(PAP)生成率为32.5%±2.5%,而pH为4时PNP降解率为80.1%±4.1%,PAP生成率为13.3%±2.2%;外接电阻为100Ω时,MFC对PNP降解性能优于外接电阻50、200Ω时。阴极优势菌群中,懒杆菌科(Ignavibacteriaceae)推动了系统电子的传递,而嗜氢菌目(Hydrogenophilales)、伯克霍尔德氏菌目(Burkholderiales)具有辅助还原降解PNP的作用。
The cathode functional microbial fuel cell (MFC) was constructed by adding the sludge into the cathode chamber,and the obtained MFC was used to strengthen the degradation of p- nitrophenol (PNP). The changes of environmental factors including pH,conductivity and temperature during the operation process were investigated.The relation between the environmental factors and the output voltage of MFC was studied by using SPSS 13.0 statistical analysis software,and the microflora structure of the cathode biofilm samples was analyzed by high-throughput sequencing. The results showed that the output voltage of MFC was extremely significantly correlated with the pH of anode and cathode chambers. The degradation rates of PNP and the formation rates of p -aminophenol (PAP) were decreased from 100.0% and 32.5%±2.5% at pH 6,to 80.1%±4.1% and 13.3%±2.2% at pH 4. While the external resistance was 100 Ω,the PNP degradation performance of MFC was superior to the resistance of 50,200 Ω. Among the cathode dominant bacteria groups, Ignavibacteriaceae could facilitate the electron transfer of the system,and Hydrogenophilales and Burkholderiales could assist in reducing nitrophenol.
作者
徐钰茜
华琮歆
徐小朋
张丽彬
沈锦优
王连军
XU Yuxi;HUA Congxin;XU Xiaopeng;ZHANG Libin;SHEN Jinyou;WANG Lianjun(School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing Jiangsu 210094)
出处
《环境污染与防治》
CAS
CSCD
北大核心
2019年第10期1177-1181,1187,共6页
Environmental Pollution & Control
基金
国家自然科学基金资助项目（No.51508275）
关键词
微生物燃料电池
环境因子
对硝基苯酚
菌群结构
microbial fuel cell
environmental factors
p- nitrophenol
microbial composition
