摘要
In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of white clover were investigated by SRAP (sequence related amplified polymorphism).Forty SRAP primers were screened,and 792 reliable bands were amplified,among which 426 (53.8%) were polymorphic.The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6.SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812,indicating that there was certain genetic diversity among white clover.Specific bands appeared in white clover variants,indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants.The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597,indicating that the different red clover germplasms had genetic diversity at the DNA molecular level.The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover.This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship,germplasm resource identification and genetic diversity of clover.
In the present study, genetic diversity and genetic relationship of 11 materials including red clover(Trifolium pretense L.), white clover(T. repen L.) and alsike clover(T. hybridum L.) and leaf-type variants of white clover were investigated by SRAP(sequence related amplified polymorphism). Forty SRAP primers were screened, and 792 reliable bands were amplified, among which 426(53.8%) were polymorphic. The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6. SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812, indicating that there was certain genetic diversity among white clover. Specific bands appeared in white clover variants, indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants. The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597, indicating that the different red clover germplasms had genetic diversity at the DNA molecular level. The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover. This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship, germplasm resource identification and genetic diversity of clover.
基金
Supported by Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016A02-1)
China Agriculture Research System-Green Manure(CARS-22)
Young Talents Training Program of Shandong Academy of Agricultural Sciences(2018-2020)
