摘要
本研究旨在探明月季花外植体在组织培养过程中不同灭菌方式及培养基对外植体的影响。结果表明:灭菌效果以乙醇(75%) 15 S+次氯酸钠(有效氯≥10%) 10 min和HgCl2(0. 1%) 10 min+双氧水(H2O23. 0%~3. 2%) 10 min最佳;MS培养基和1/2 MS培养基对外植体的生长影响不大;乙醇(75%) 15 S和次氯酸钠(有效氯≥10%) 10 min和HgCl2(0. 1%) 10 min+双氧水(H2O2 3. 0%~3. 2%) 10 min对月季茎段根的生长影响最小。
This study aims to find out the impact on explants caused by different sterilization methods and medium during tissue culture Rosa chinensis. Experimental result showed that the best sterilization effect was caused by ethanol( 75 %) 15 S + sodium hypochlorite( available chlorine≥10 %) 10 min and HgCl2( 0. 1 %) 10 min + H2 O2( 3. 0 %-3. 2 %) 10 min;Next came MS medium and 1/2 MS medium;Ethanol( 75 %) 15 S + sodium hypochlorite( available chlorine≥10 %) 10 min and HgCl2( 0. 1 %) 10 min + H2O2( 3. 0 %-3. 2 %) 10 min has the least influence on the growth of china rose’s stem and root segment.
作者
何燕
任盼盼
段瑜
HE Yan;REN Pan-pan;DUAN Yu(Plant Science Colleae,Tibet Agriculture&Animal Husbandry University,Tibet Linzhi 860000,China)
出处
《西藏农业科技》
2019年第3期11-15,共5页
Tibet Journal of Agricultural Sciences
基金
“植物组培产业化”开发与示范推广
关键词
灭菌方式
灭菌效果
月季花
Sterilization methods
Germedical effect
Monthly rose
