摘要
目的观察维生素B12(VB12)联合鼠神经生长因子(MNGF)对脑瘫幼鼠智力的改善作用,并探讨其机制。方法选取14 d日龄Wistar大鼠60只,随机分为5组,即对照组、模型组、VB12组、MNGF组、VB12+MNGF组,各12只。除对照组外均建立脑瘫幼鼠模型。VB12组给予VB12注射液(以无菌生理盐水配置,终浓度为70 mg/100 ml)10 ml/kg腹腔注射,每天1次;MNGF组给予MNGF注射液0.2 ml腹腔注射,每天1次;VB12+MNGF组干预方法与VB12组和MNGF组相同,对照组和模型组均给予等量无菌生理盐水。各组幼鼠均干预21 d,对比各组一般情况变化,Y迷宫实验结果,大脑皮质神经细胞凋亡指数(AI),脑皮质中央前回区组织天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)、B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白基因(Bax)mRNA及蛋白表达。结果对照组大鼠饮食、转圈、行走、日常活动、睡眠、大小便等均正常,且体质量呈正常增长趋势,对照组状态最佳,VB12+MNGF组次之,MNGF组稍差,VB12组更差,模型组最差;各组幼鼠Y迷宫实验正确次数、平均开灯至逃至安全区所用时间、大脑皮质神经细胞AI、脑皮质中央前回区组织Caspase-3、Bcl-2、Bax mRNA及蛋白对比差异均有统计学意义(P<0.05),且每2组间比较差异均有统计学意义(P<0.05),其中对照组正确次数最多,VB12+MNGF组次之,MNGF组稍少,VB12组更少,模型组最少;模型组平均开灯至逃至安全区所用时间最长,VB12组次之,MNGF组稍短,VB12+MNGF组更短,对照组最短;模型组大脑皮质神经细胞AI、脑皮质中央前回区组织Caspase-3、Bax mRNA及蛋白均最高,VB12组均次之,MNGF组均稍低,VB12+MNGF组均更低,对照组均最低;对照组Bcl-2 mRNA及蛋白均最高,VB12+MNGF组均次之,MNGF组均稍低,VB12组均更低,模型组均最低。结论对脑瘫幼鼠实施VB12联合MNGF干预能够显著改善一般状况和智力,减少大脑皮质神经细胞凋亡,推测很可能是通过下调Caspase-3、Bax mRNA及
Objective To investigate the effect of vitamin B12 (VB12) combined with mouse nerve growth factor (MNGF) on the intelligence improvement in rats with cerebral palsy rats and the related mechanism. Methods Sixty 14-day-old Wistar rats were randomly divided into 5 groups: control group, model group, VB12 group, MNGF group and VB12+MNGF group (n=12 each). Cerebral palsy was modeled in all rats except for the control group. The VB12 group was given intraperitoneal injection of 10 ml/kg VB12 (added with sterile normal saline to a final concentration of 70 mg/100 ml) once daily;the MNGF group was given intraperitoneal injection of 0.2 ml MNGF once daily;the VB12+MNGF group received combined intervention as in the VB12 group and the MNGF group;the control group and the model group were given the equivalent amount of sterile normal saline. All the young rats were intervened for 21 days, and were then compared for the general conditions, results of Y-maze test, cerebral cortex apoptosis index (AI), and the mRNA and protein expression of aspartate-specific cysteine protease-3 (Caspase-3), B cell lymphoma/leukemia-2 (Bcl-2), Bcl-2 related X protein (Bax) in the prefrontal cortex brain tissues. Results The control group were normal in dieting, rotation behaviors, walking, daily activities, sleep, urination and defecation. The body weight showed a tendency of natural growth in all groups, which was most eminent in the control group, followed by VB12+MNGF group, MNGF group, VB12 group, and the model group. The number of correct response and the time spent running to the safety zone with light on in the Y maze test, cerebral cortex AI, and the mRNA and protein expression of Caspase-3, Bcl-2 and Bax in the prefrontal cortex brain tissues statistically significant among groups (P<0.05) and between groups (P<0.05). The control group showed the highest number of correct response, followed by the VB12+MNGF group, MNGF group, VB12 group and model group. The model group had the longest time to escape to the safety zone with light on,
作者
鲁玉霞
杨淑明
Lu Yuxia;Yang Shuming(Department of Children Rehabilitation, Puyang Maternal and Children’s Hospital, Puyang, Henan 457000, China)
出处
《中华生物医学工程杂志》
CAS
2019年第3期360-366,共7页
Chinese Journal of Biomedical Engineering
关键词
维生素B12
鼠神经生长因子
脑瘫幼鼠
智力
机制
Vitamin B12
Mouse nerve growth factor
Cerebral palsy
Young rats
Intelligence
Mechanism
