摘要
目的通过动物实验评价续骨油湿敷剂的抗炎、镇痛作用及其安全性。方法SD大鼠50只,随机分为对照组、扶他林组、续骨油湿敷剂高、中、低剂量组(n=10),注入蛋清前后各涂药1次,在注入蛋清前及注入后0.5、1、2、4、6h观察大鼠肿胀肢体厚度,以研究续骨油湿敷剂对蛋清所致大鼠足趾肿胀的影响,评价其抗急性炎性反应作用。NIH小鼠100只,随机分为二甲苯致炎实验组、冰醋酸扭体实验组,各组再分别分为对照组、扶他林组、续骨油湿敷剂高、中、低剂量组(n=10),每日给药1次,连续3d。二甲苯致炎实验组在末次给药1h后涂抹二甲苯以致炎,30min后处死小鼠研究续骨油湿敷剂对二甲苯所致小鼠耳廓肿胀的影响,评价其抗炎作用;醋酸扭体实验组在末次给药1h后,腹腔注射醋酸0.2ml/只,观察15min内小鼠的扭体情况以评价续骨油湿敷剂对冰醋酸所致小鼠扭体的镇痛作用。背部去毛的白化豚鼠32只,随机分为单次给药组和多次给药组,各组又分别分为完整皮肤组、破损皮肤组(n=8)。单次给药组用药24h洗除药液,分别于1、24、72h观察皮肤;多次给药组每日给药3次,连续7d,停药后观察皮肤7d,以研究续骨油湿敷剂对豚鼠完整及破损皮肤的影响,评价其外用的安全性。结果与对照组相比,扶他林组、续骨油湿敷剂高、中、低剂量组在给药后0.5、1h大鼠足趾肿胀度降低(均P<0.05)。与对照组相比,扶他林组、续骨油湿敷剂高、中剂量小鼠耳廓肿胀度降低(均P<0.05)。与对照组相比,扶他林组、续骨油湿敷剂高、中、低剂量组扭体次数降低(均P<0.05)。单次给药实验中完整皮肤组及破损皮肤组豚鼠在涂药后1、24、72h均存活,其饮食、行为活动、眼和黏膜、精神状态、粪便及分泌物均正常,皮肤无明显红斑、无水肿现象。多次给药实验中,完整皮肤组及破损皮肤组豚鼠在用药的7d及停药观察7d中均存活,其
Objective To evaluate the anti-inflammatory and analgesic effects and the safety profiles of Xugu Oil Moist Compress (XOMC) via animal experiments. Methods Fifty SD rats were randomly divided into control group, Voltaren group and XOMC high-, medium- and low-dose groups (n=10 each). The animals received skin application of corresponding agents before injection of egg white. The thickness of the swollen rat limbs was measured at baseline and at 0. 5, 1, 2, 4, and 6 h after the injection, such that the effect of XOMC on egg white induced toe swelling in rats and its action against acute inflammation were evaluated. A total of 100 NIH mice were randomly divided into the xylene-induced inflammation group and glacial acetic acid writhing group. Each of these two groups was further divided into the control group, Voltaren group and XOMC high-, medium- and low-dose groups (n=10 each) to be applied with corresponding agents once daily for 3 consecutive days. In the xylene-induced inflammation group, the mice were applied with xylene to induce inflammation at 1 h after the last skin administration, and sacrificed 30 min later, such that the effect of XOMC on xylene-induced auricle swelling and its anti-inflammatory action were evaluated. In acetic acid writhing group, the mice were intraperitoneally injected with acetic acid (0.2 ml each) at 1 h after the last skin administration, such that the writhing of the mice within 15 min following the injection, and the analgesic effect of XOMC on glacial acetic acid induced writhing were determined. Thirty-two back-shaved albino guinea pigs were randomized into the single-dose group and the multiple-dose group. Each of these two groups was divided into the skin-intact group and skin-damaged group (n=8 each). The mice in the single-dose group were washed off the topical XOMC at 24 h after application, and observed for the skin condition at 1, 24, and 72 h. The mice in the multiple-dose group received skin administration of XOMC 3 times daily for 7 days, and were subject to a 7-day
作者
冯小映
林静吟
李嘉华
孙玉兴
Feng Xiaoying;Lin Jingyin;Li Jiahua;Sun Yuxing(Department of Pharmacy, Guangzhou Orthopedics Hospital, Guangzhou 510045, China;Guangdong Institute of Traditional Chinese Medicine Engineering, Guangzhou 510095, China)
出处
《中华生物医学工程杂志》
CAS
2019年第3期269-273,共5页
Chinese Journal of Biomedical Engineering
基金
广东省中医药局立项科研课题(20152045).
关键词
续骨油湿敷剂
抗炎剂(中药)
镇痛
安全性评价
Xugu Oil Moist Compress
Anti-inflammatory agent (TCD)
Analgesia
Safety evaluation
