摘要
为确定导致西藏那曲地区牦牛发病死亡的细菌性病原,采集病死牦牛内脏进行细菌学检测,分离出13株巴氏杆菌疑似菌,应用巴氏杆菌实时荧光定量PCR法对分离菌进行鉴定,并检测分离菌对常用30种抗菌药物的敏感性。试验结果显示:13株菌经实时荧光定量扩增后,Ct值≤35.0,且有明显扩增曲线,均为多杀巴氏杆菌;分离菌仅对克林霉素和万古霉素两种表现耐药,其中克林霉素的耐药率为23.08%,万古霉素的耐药率为69.23%;通过对引起牦牛死亡的细菌性病原的耐药情况分析,筛选出了对牦牛巴氏杆菌敏感的药物,为指导临床科学用药和防控提供依据。
In order to identify the bacterial pathogens causing yak morbidity and death in Naqu Tibet, 13 suspected Pasteurella strains were isolated from the viscera of dead yaks and identified by real-time fluorescence quantitative PCR, and then the sensitivity of the isolated bacteria to 30 commonly used antibiotics was tested.The results showed that after real-time fluorescence quantitative amplification, the CT value of 13 strains of bacteria was less than 35.0, but the amplification curve was obvious, showing that all of them were Pasteurella multocida.The isolates were resistant to clindamycin and vancomycin only, of which clindamycin resistance rate was 23.08% and vancomycin resistance rate was 69.23%.Drugs susceptible to Pasteurella in yaks were developed, which provided basis for clinical scientific drug use and control.
作者
陈建春
王一飞
周赛赛
罗润波
贡嘎
曲久
高家登
索朗斯珠
CHEN Jianchun;WANG Yifei;ZHOU Saisai;LUO Runborn;Gongga;Qujiu;GAO Jiadeng;Suolang sizhu(Animal Science College,Tibet Agriculture&Animal Husbandry University,Nyingchi Tibet 860000;Veterinary Station of Naqu City,Naqu Tibet,852002,China)
出处
《高原农业》
2019年第5期515-521,483,共8页
Journal of Plateau Agriculture
基金
国家肉牛牦牛产业体系项目（CARS-37）
西藏科技厅重大项目资助
关键词
牦牛
多杀巴氏杆菌
荧光定量检测
药敏试验
yak
Pasteurella multocida
fluorescence quantitative detection
in vitro antimicrobial susceptibility test
