摘要
目的分析TOLL受体4(TLR4)和NF-κB在子痫前期(PE)患者血清中的表达、与临床病理特征的关系及其作为标记物诊断PE的临床价值。方法选择江陵县人民医院收治的PE患者15例,同期正常妊娠者20例作为研究对象。采用ELISA方法检测两组人群血清TLR4、NF-κB蛋白浓度。比较两组人群中血清TLR4、NF-κB蛋白水平差异。根据贝叶斯定理,评价血清TLR4、NF-κB为标志物诊断PE的临床效能。结果PE组患者血清TLR4与NF-κB水平显著高于正常妊娠组(P<0.05),且两者存在正相关(r=0.45,P<0.05),随着TLR4水平的升高,血清NF-κB水平显著上升;分别以血清TLR4和NF-κB为参考指标诊断PE的敏感性分别为68.9%和72.3%,特异性分别为70.2%和76.4%。诊断的ROC曲线下面积分别为0.92和0.95。结论血清TLR4和NF-κB蛋白水平在PE患者中显著升高,可能参与了PE的发病过程,并可作为诊断PE的血清学标志物。
Objective The purpose was to assess the evaluate the clinical significance of NF-κB and TLR4 expression in patients with preeclampsia. Methods Fifteen patients with preeclampsia and 20 healthy control were included in this study. The serum level of NF-κB and TLR4 were assayed by ELISA, and compared between the two groups. Further diagnosis value for NF-κB and TLR4 were evaluated according to Bey's theorem. Results The serum level of NF-κB and TLR4 were significant higher in preeclampsia patients compared to healthy control(P < 0.05). The diagnostic sensitivity and specificity were 68.9% and 70.2% for serum TLR4 and 72.3% and 76.4% for serum NF-κB with AUC of 0.92 and 0.95 respectively. Conclusion Serum levels of TLR4 and NF NF-kappa-B protein level was significantly higher in patients with preeclampsia which may involved in the pathogenesis of preeclampsia,and can be used as a marker for the serological diagnosis of preeclampsia.
作者
王玲
WANG Ling(Departmet of Obstetrics and Gynecology,Jiangling people's Hospital,Jingzhou City,Hubei 434101,China)
出处
《中国处方药》
2019年第10期161-162,共2页
Journal of China Prescription Drug
