摘要
目的探讨纳米材料羧甲基石墨烯-精胺(CGS)用于抗生殖系统肿瘤的作用。方法配置成浓度为20、40、60、80和100μg/ml的CGS液,对照组CGS浓度为0μg/ml。利用傅里叶变红外光谱、扫描电子显微镜X射线衍射和原子力显微镜来检测了CGS的物化学特性;并以卵巢癌细胞(Skov3)为细胞模型,检测CGS的抗肿瘤活性。结果通过FTIR检测技术发现,CGS吸收峰在3415.42cm-1、1629.37cm-1、1402.08cm-1、1058.39cm-1有4处吸收峰。XRD图谱表明,CGS在10.6°时有且只有一个峰,表明CGS复合纳米材料中没有其它杂质。SEM表明相邻的石墨烯层被隔开,有很好的分散性。AFM展示了良好分散的CGS形态学特征,这些颗粒的粒径大约分布在87nm左右。随着药物浓度的升高,Skov3细胞活性随之下降。CGS(浓度为20、40、60、80、100μg/ml)处理24h后,Skov3细胞的活性相比于对照组分别下降到81%、82%、77%、75%和66%,100μg/ml时最为显著(P均<0.05)。但100μg/ml浓度处理48h与24h相比较,细胞活性变化不显著(P>0.05)。细胞内活化氧(ROS)随给药浓度升高而提高。结论CGS具有可调控的微纳结构和宏观形态;CGS可抑制Skov3细胞的增殖,引起ROS的增加,并诱导细胞的线粒体膜电位降低。此为CGS用于生殖系统疾病的治疗奠定了良好的基础。
Objective:To study the effect of nanomaterials carboxymethyl graphene-spermine(CGS)on anti-reproductive system tumors.Methods:CGS solutions were prepared at concentrations of 20,40,60,and 80μg/ml,and the control group had a CGS concentration of 0μg/ml.The physicochemical properties of CGS were detected by Fourier variable infrared spectroscopy,scanning electron microscope X-ray diffraction,and atomic force microscope.And the Skov3 cell was used as a model to detect the anticancer activity of CGS.Results:The FTIR detection technique found that the CGS absorption peaks had four absorption peaks at 3 415.42 cm-1,1 629.37 cm-1,1 402.08 cm-1,and 1 058.39 cm-1.The XRD pattern shows that CGS had one and only one peak at 10.6°,indicating that there were no other impurities in the CGS composite nanomaterial.The SEM showed that the adjacent graphene layers were separated and had good dispersibility.AFM exhibited well-distributed CGS morphological features,and the particle size of these particles was approximately 87 nm.As the concentration of the drug increases,the activity of Skov3 cells was decreased.After treatment with CGS at 20,40,60,80,100μg/ml for 24 hours,the activity of Skov3 cells was decreased to 81%,82%,77%,75%and 66%,respectively,compared with the control group,and the most significant decrease was observed in 100μg/ml(P<0.05).However,the cell activity was not significantly different between the CGS of 100μg/ml treatment for 24 hours and for 48 hours(P>0.05).ROS increased along with increasing concentration of CGS.Conclusions:CGS has a controllable micro-nano structure and macroscopic morphology.CGS inhibits the proliferation of Skov3 cells,causes an increase in the intracellular activated oxygen(ROS),and induces a decrease in mitochondrial membrane potential.This lays a good foundation for using CGS to treat reproductive diseases.
作者
朝呼和
徐长远
张西锋
CHAO Hu-he;XU Chang-yuan;ZHANG Xi-feng(Central Laboratory,Beijing Obstetrics & Gynecology Hospital,Capital Medical University,Beijing100026;College of Biological & Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan430023)
出处
《生殖医学杂志》
CAS
2019年第10期1227-1231,共5页
Journal of Reproductive Medicine
基金
国家自然科学基金(21602166)
关键词
卵巢癌
石墨烯
线粒体膜电位
Graphene
Ovarian cancer cells
Mitochondrial membrane potential
