摘要
目的比较IVF3PN、IVF2PN、ICSI3PN和ICSI2PN胚胎的卵裂模式和胚胎动力学参数的差异。方法回顾性分析2017年7月至2018年6月在我院生殖中心就诊经时差胚胎监测(Time-lapse)培养箱培养的393个胚胎发育动力学参数,根据受精结局分为4组:IVF3PN组(n=132)、IVF2PN组(n=150)、ICSI3PN组(n=11)和ICSI2PN组(n=100),分析比较各组胚胎的卵裂模式和原核出现时间、原核消失时间、第一次卵裂时间、t4、t3-t5等胚胎动力学参数。结果IVF3PN组胚胎第一次卵裂模式异常率(100%)显著高于IVF2PN组胚胎(4.67%)、ICSI3PN组胚胎(18.18%)和ICSI2PN组胚胎(5.00%)(P<0.001),而ICSI3PN组、ICSI2PN组和IVF2PN组胚胎之间的第一次卵裂模式异常率无显著差异(P>0.05)。IVF3PN组胚胎原核消失时间[(25.76±1.33)h]显著长于IVF2PN组[(21.43±1.76)h]、ICSI3PN组[(21.86±1.63)h]和ICSI2PN组[(20.14±1.78)h](P<0.05),IVF3PN胚胎第一次卵裂时间亦显著长于其余3组[(27.06±1.59)hvs.(22.64±1.38)h、(22.32±1.56)h、(22.78±1.66)h](P<0.05);ICSI3PN组、ICSI2PN组和IVF2PN组胚胎之间的原核消失时间和第一次卵裂时间均无显著差异(P>0.05)。结论IVF3PN胚胎与IVF2PN、ICSI3PN和ICSI2PN胚胎的卵裂模式和原核消失时间、第一次卵裂时间等动力学参数不同。
Objective:To compare the cleavage pattern and embryonic dynamics among embryos of IVF 3PN,IVF 2PN,ICSI 3PN and ICSI 2PN.Methods:A retrospective analysis was performed on the 393 embryos developmental kinetics parameters cultured in Time-lapse incubator in the reproductive center of our hospital from July 2017 to June 2018.According to the fertilization outcome,these embryos were divided into four groups:IVF 3PN group(n=132),IVF 2PN group(n=150),ICSI 3PN group(n=11),and ICSI 2PN group(n=100).The embryo cleavage pattern and embryo dynamics parameters including prokaryotic appearance time,prokaryotic disappearance time,first cleavage time,t4,t3-t5 were compared among the groups.Results:The abnormal rate of first cleavage pattern in IVF 3PN group(100%)was significantly higher than that in IVF 2PN group(4.67%),ICSI 3PN group(18.18%)and ICSI 2PN group(5.00%)(P<0.001).There was no significant difference in the abnormal rate of first cleavage pattern among ICSI 3PN group,ICSI 2PN group and IVF 2PN group(P>0.05).The prokaryotic disappearance time of IVF 3PN group[(25.76±1.33)h]was significantly longer than that of IVF 2PN group[(21.43±1.76)h],ICSI 3PN group[(21.86±1.63)h]and ICSI 2PN group[(20.14±1.78)h](P<0.05).The first cleavage time of IVF 3PN embryo[(27.06±1.59)h]was significantly longer than that of IVF 2PN group[(22.64±1.38)h],ICSI 3PN group[(22.32±1.56)h]and ICSI 2PN group[(22.78±1.66)h](P<0.05).There was no significant difference in prokaryotic disappearance time and first cleavage time among ICSI 3PN group,ICSI 2PN group and IVF 2PN group(P>0.05).Conclusions:The cleavage patterns,prokaryotic disappearance time,first cleavage time and other kinetic parameters are different between IVF 3PN embryos and IVF 2PN,ICSI 3PN and ICSI 2PN embryos.
作者
史艳彬
马超
余巧巧
邵小光
SHI Yan-bin;MA Chao;YU Qiao-qiao;SHAO Xiao-guang(Reproductive & Genetic Medicine Center,Dalian Women’s & Children’s Medical Center,Dalian 116000)
出处
《生殖医学杂志》
CAS
2019年第10期1158-1161,共4页
Journal of Reproductive Medicine
基金
辽宁省自然科学基金面上项目引导计划(201800937)
