摘要
目的:探讨miR-21在胃癌细胞系中对SOX2的调控作用及其对胃癌细胞迁移的影响。方法:在胃癌细胞系AZ-521、AGS中通过转染miR-21mimic或者miR-21antagomir构建miR-21过表达和低表达模型,RT-qPCR检测转染效果,并通过蛋白免疫印迹法检测SOX2在转染细胞中表达的变化。构建miR-21双荧光素酶报告基因,在工具细胞HEK293T中检验miR-21是否通过直接结合SOX2的3′-UTR区域来对其实现调控。应用Transwell迁移实验检测miR-21及SOX2对胃癌细胞迁移能力的影响。结果:miR-21直接结合于SOX2的3′-UTR区域来抑制SOX2在胃癌细胞系中的表达。迁移实验发现上调miR-21或者下调SOX2都能促进胃癌细胞的迁移。结论:miR-21可促进胃癌细胞的迁移能力,其机制可能通过抑制SOX2的表达而实现。
Objective:To investigate the regulation of miR-21 on SOX2 in gastric cancer cell lines and its effect on the migration of gastric cancer cells.Methods:AZ-521 and AGS were transfected with mimic or antagomir to increase or decrease miR-21 expression.The expression of miR-21 was detected by RT-qPCR and the expression of SOX2 was detected by Western blot.To test whether miR-21 can regulate SOX2 by directly binding to its 3'-UTR region.A double luciferase reporter gene of miR-21 was constructed in HEK293T.Transwell assay was used to detect the effects of miR-21 and SOX2 on gastric cancer cell migration.Results:The expression of SOX2 in gastric cancer cell lines was inhibited by the direct binding of miR-21 to the 3'-UTR region of SOX2.We also demonstrated that either up-regulation of miR-21 or down-regulation of SOX2 could promote the migration of gastric cancer cells.Conclusion:miR-21 directly regulates the expression of SOX2 and affects the migration of gastric cancer AGS and AZ-521 cells.
作者
袁挺
韩川
孙妮娜
刘彩芳
石淼
时永全
Yuan Ting;Han Chuan;Sun Nina;Liu Caifang;Shi Miao;Shi Yongquan(State Key Laboratory of Cancer Biology,National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases,Airforce Military Medical University,Shaanxi Xi'an 710032,China;The 150th Hospital of the People's Liberation Army,Henan Luoyang 471000,China;Rocket Army Emei Sanatorium,Sichuan Emei 614200,China;College of Postgraduates,Xi'an Medical University,Shaanxi Xi'an 710021,China)
出处
《现代肿瘤医学》
CAS
2019年第19期3361-3364,共4页
Journal of Modern Oncology
基金
国家自然科学基金(编号:81873554,81470805)
陕西省创新能力支撑计划(编号:2018TD-003)
