摘要
为半夏种苗繁育提供参考,采用组织培养法,通过单因素试验和3因素4水平正交试验,探索6-BA、NAA、2,4-D生长调节剂不同浓度及其组合对圆珠半夏顶芽萌发、丛生芽诱导和愈伤组织诱导的影响。结果表明:不同浓度6-BA、NAA、2,4-D及其组合对顶芽萌发、愈伤组织和丛生芽诱导具有显著或极显著促进作用。单因素试验中,1.0mg/L6-BA、0.2mg/LNAA、0.2mg/L2,4-D诱导的愈伤组织块数最多,分别为26块、29块和24块。以圆珠半夏带顶芽块茎为外植体进行组织培养时,愈伤组织形成的最优组合为MS+6-BA1.0mg/L+NAA0.3mg/L+2,4-D0.3mg/L,诱导顶芽萌发和丛生芽形成的最优组合为MS+6-BA1.2mg/L+NAA0.3mg/L+2,4-D0.2mg/L。
To provide references for Pinellia ternata seedling breeding,the effects of 6-BA,NAA and 2,4-D on apical bud germination and cluster bud induction were studied by means of tissue culture and orthogonal experiment with three factors and four levels.Results:Different concentrations of 6-BA,NAA,2,4-D and their combinations had significant or extremely significant effects on apical bud germination,callus induction and cluster bud induction.In single factor experiment,callus blocks induced by 1.0 mg/L 6-BA,0.2 mg/L NAA,0.2 mg/L 2,4-D were the most,which were 26,29 and 24,respectively.When the tubers of P.ternata with apical buds were used as explants for tissue culture,the optimal combination for callus induction was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L+2,4-D 0.3 mg/L.The optimum combination of inducing apical bud germination and cluster bud formation was MS+6-BA 1.2 mg/L+NAA 0.3 mg/L+2,4-D 0.2 mg/L.
作者
朱健
罗夫来
赵致
王华磊
罗春丽
李金玲
刘红昌
黄明进
ZHU Jian;LUO Fulai;ZHAO Zhi;WANG Hualei;LUO Chunli;LI Jinling;LIU Hongchang;HUANG Mingjin(Agricultural College,Guizhou University,Guiyang,Guizhou 550025;Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guiyang,Guizhou 550025,China)
出处
《贵州农业科学》
CAS
2019年第9期118-121,共4页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目“基于化感自毒作用理论的半夏连作障碍机理研究”（81460577）
贵州省科技计划项目“圆珠半夏生态栽培模式研究与示范应用”[黔科合支撑（2018）2825]
贵州省科技创新人才团队建设项目“贵州省中药材资源保护与可持续利用研究创新团队”[黔科合人才团队（2010）4006]
关键词
圆珠半夏
带顶芽块茎
诱导
愈伤组织
丛生芽
快速繁殖
Pinellia ternata
tubers with apical buds
induction
callus
cluster buds
rapid propagation
