摘要
研究宁夏枸杞(Lycium barbarum L.)根部和茎部的化学成分。采用硅胶柱、ODS开放柱、Sephadex LH-20葡聚糖凝胶柱及半制备反相高效液相等色谱手段,对宁夏枸杞根和茎部乙醇提取物的石油醚部位及乙酸乙酯部位化学成分进行分离纯化,根据其理化性质以及波谱数据鉴定得到12个化合物,分别为N-[2-(3,4-dihydroxyphenyl)-2-hydroxyethyl]-3-(4-methoxyphenyl)prop-2-enamide(1)、3-(4-hydroxy-3-methoxy phenyl)-N-2-(4-hydroxyphenyl)-2-methoxyethyl]acrylamide(2)、N-trans-coumaroyloctopamine(3)、(E)-2-(4,5-dihydroxy-2-{3-[(4-hydroxyphenethyl)amino]-3-oxopropyl}phenyl)-3-(4-hydroxy-3,5-dimethoxyphenyl)-N-(4-acetamidobutyl)acrylamide(4)、1,2-dihydro-6,8-dimethoxy-7-hydroxy-1-(3,4-dihydroxy-phenyl)-N1,N2-bis[2-(4-hydroxyphenyl)ethyl]-2,3-naphthalene dicarboxamide(5)、(+)-syringaresinol(6)、zhebeiresinol(7)、(±)-eriodictyol(8)、isovanilin(9)、5,5′-dimethoxybiphenyl-2,2′-diol(10)、p-hydroxyphenethyltrans-ferulate(11)、E-ferulic acid hexacosyl ester(12),所有化合物均为首次从该植物中分离得到。此外,采用MTT法和抑制一氧化氮(NO)生成实验,从细胞毒活性和抗炎活性两方面评估了化合物的生物活性。结果表明,化合物2具有显著的抗炎活性,其IC50值(17.00±1.11μmol/L)小于阳性对照药槲皮素的IC50值(17.21±0.50μmol/L)。
The chemical constituents in ethyl acetate and petroleum ether fractions of the ethanol extract from root and stem of Lycium barbarum L.were separated and purified by using silica gel,ODS and Sephadex LH-20 column chromatography in addition to reversed phase semi-preparative HPLC.According to the physicochemical properties and spectroscopic data,the compounds were identified.As a result,twelve compounds were obtained and identified as N -[2-(3,4-dihydroxyphenyl)-2-hydroxyethyl]-3-(4-methoxyphenyl)prop-2-enamide ( 1 ),3-(4-hydr-oxy-3-methoxyphenyl)- N -[2-(4-hydroxyphenyl)-2-methoxyethyl] acrylamide ( 2 ),N - trans -coumaroyloctopamine ( 3 ),( E )-2-(4,5-dihydroxy-2-{3-[(4-hydroxyphenethyl)ami no]-3-oxopropyl}phenyl)-3-(4-hydroxy-3,5-dimethoxyp-henyl)- N -(4-acetamidobutyl)acrylamide ( 4 ),1,2-dihydro-6,8-dimethoxy-7-hydroxy-1-(3,4-dihydroxyphenyl)- N 1,N 2-bis[2-(4-hydroxyphenyl)ethyl]-2,3-naphthalene dicarboxamide ( 5),(+)-syringaresinol (6),zhebeiresinol (7),(±)-eriodictyol (8),isovanilin (9 ),5,5′-dimethoxybiphenyl-2,2′-diol ( 10),p -hydroxyphenethyl trans -ferulate (11),E -ferulic acid hexacosyl ester (12 ).All the compounds were isolated from this plant for the first time.The cytotoxic and anti-inflammatory activities of the compounds were evaluated by using MTT assay and nitric oxide (NO) production inhibition experiment.It was turned out that compound 2 showed the IC 50 value 17.00 ± 1.11 μmol/L) due to the significant anti-inflammatory activity,which was lower than that of quercetin (IC 50 = 17.21 ± 0.50 μmol/L).
作者
兰婷
黄远鹏
梁秋萍
周光雄
LAN Ting;HUANG Yuan-peng;LIANG Qiu-ping;ZHOU Guang-xiong(Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research,Institute of Traditional Chinese Medicine and Natural Product,College of Pharmacy,Jinan University,Guangzhou 510632,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第9期1491-1497,共7页
Natural Product Research and Development
基金
国家自然科学基金面上项目(81573578)
国家中医药局项目(2017-110108-83-03-001423)
关键词
宁夏枸杞
根和茎
化学成分
分离纯化
Lycium barbarum L.
root and stem
chemical constituents
separation and purification
