摘要
从内蒙古酸马奶中分离一株对鼠伤寒沙门氏菌ATCC14028具有强抑菌活性的菌株MXG-68,经个体形态、菌落形态、16S rDNA基因同源性比对及系统进化树构建,确定该菌株为植物乳杆菌。水解酶敏感性试验结果显示植物乳杆菌MXG-68所产抑菌物质为蛋白质(多肽)。通过单因素试验确定温度、初始pH、抗坏血酸、EDTA对抑菌活性影响的基础上,应用响应面法的Box-Behnken设计进行植物乳杆菌MXG-68产细菌素发酵条件的优化。当温度30.08℃、初始pH 6.94、抗坏血酸1.91μg/mL、EDTA 5.06mg/mL时可获得最大的抑菌圈直径21.42 mm,与优化前的溶圈直径相比提高46.41%,表明该模型能科学地预测细菌素的合成情况。
A strain with strong inhibition activity against Salmonella typhimurium ATCC14028 was isolated from Inner Mongolia traditional fermented koumiss. Strain MXG-68 was identified as Lactobacillus plantarum by individual morphology, colony morphology, homology comparison of 16 S rDNA, and construction of phylogenetic tree. The results of hydrolase sensitivity test demonstrated that the antimicrobial substance produced by Lactobacillus plantarum MXG-68 was a protein(polypeptide). On the basis of determining the effects of temperature, initial pH, ascorbic acid, and EDTA on the antibacterial activity, Box-Behnken design of Response Surface Methodology was used to optimize the fermentation conditions of Lactobacillus plantarum MXG-68 for bacteriocin production. The maximal inhibition diameter of bacteriocin in actual experiments was 21.42 mm with 30.08 ℃, initial pH 6.94, scorbic acid 1.91 μg/mL, and EDTA 5.06 mg/mL, and the inhibition diameter had been increased by 46.41 % as compared to that of un-optimized conditions. It was demonstrated that the use of Response Surface Methodology can effectively and successfully optimize the fermentation conditions of bacteriocin production.
作者
满丽莉
向殿军
MAN Lili;XIANG Dianjun(College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028042, China;College of Agricul-ture, Inner Mongolia University for Nationalities, Tongliao 028042, China)
出处
《中国乳品工业》
CAS
北大核心
2019年第8期4-9,25,共7页
China Dairy Industry
基金
内蒙古自治区自然科学基金资助(2018MS03060)
内蒙古民族大学博士科研启动基金资助(BS403),内蒙古民族大学实验室开放项目
关键词
细菌素
筛选
植物乳杆菌
发酵条件
Bacteriocin
Screening
Lactobacillus plantarum
Fermentation conditions
