摘要
目的观察不同脂联素亚型在冠心病患者体内由全身到局部的分布变化.方法选取冠心病(CAD)患者与非冠心病(Non-CAD)患者各30例.术前抽取静脉血,术中留取心外膜和皮下脂肪组织.酶联免疫吸附试验(ELISA)检测血清总脂联素水平;凝胶过滤联用ELISA法检测血清中不同亚型脂联素的含量;实时定量反转录聚合酶链反应(RT-qPCR)检测心外膜脂肪中脂联素的表达变化;采用不加热非还原法十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法检测心外膜脂肪中脂联素亚型的分布变化.应用SPSS 15.0统计软件分析,组间对比采用非独立性t检验和方差分析.结果与Non-CAD组比较,CAD组中血清高聚体(HMW)脂联素显著降低[HMW型脂联素:(0.55±0.18)mg/L比(0.31±0.12)mg/L,t=3.472,P<0.01];而中(MMW)、低(LMW)聚体脂联素无明显降低[MMW:(0.41±0.17)mg/L比(0.43±0.16)mg/L,t=1.834,P>0.05;LMW:(0.48±0.22)mg/L比(0.53±0.20)mg/L,t=1.712,P>0.05];CAD组心外膜脂肪中脂联素mRNA的表达显著降低(1.102±0.135比0.810±0.101,t=3.351,P<0.01).在CAD组内,心外膜脂肪中脂联素mRNA表达较皮下组织显著降低(0.810±0.101比1.008±0.146,t=3.363,P<0.01).通过蛋白组检测,CAD组HMW含量显著下降(与Non-CAD组心外膜脂肪比较和自身皮下组织组比较),其他两型差异无统计学意义(P>0.05).结论冠心病患者血清及心外膜脂肪中脂联素含量的下降以HMW亚型降低为主.
Objective To study the difference of the secretion and synthesis of adiponectin in different parts of adipose tissue in patients with coronary heart disease(CHD).Methods The serum,epicardial adipose tissue(EAT)and subcutaneous adipose tissue(SAT)in over 50-years old male patients with CHD or valvular heart disease as control group.The patients with CHD were excluded by coronary angiography in control group.Serum adiponectin concentrations were examined in CHD group and control group by enzyme linked immunosorbent assay(ELISA).Serum adiponectin types were classified in accordance with the molecular weight of high-molecular-weight(HMW),medium molecular weight(MMW)and low molecular weight(LMW)in CHD group and non-CHD group by gel filtration chromatography.Real-time PCR was used to detect the expression of adiponectin mRNA in epicardial adipose cells and subcutaneous adipose cells.The levels of HMW MMW LMW adiponectin in adipose cells were determined by non-hearting and non-reducing sodium dodecyl sulfate polyacrylamide gel electropheresis(SDS-PAGE).Results As compared with non-CHD group,the concentration of serum HMW in CHD group was significantly reduced[(0.55±0.18)mg/L vs.(0.31±0.12)mg/L,t=3.472,P<0.01].while no reduction was observed in group of MMW and LMW[MMW:(0.41±0.17)mg/L vs.(0.43±0.16)mg/L,t=1.834,P>0.05;LMW:(0.48±0.22)mg/L vs.(0.53±0.20)mg/L,t=1.712,P>0.05].The expression of adiponectin mRNA in CAD epicardial group was significnatly downregulated when compared with autologous subcutaneous group(0.810±0.101 vs.1.102±0.135,t=3.351,P<0.01)and control epicardial group(0.810±0.101 vs.1.008±0.146,t=3.363,P<0.01).The proteomics revealed that the HMW content in CHD group decreased significantly as compared with non-CHD epicardial group and subcutaneous tissue group,and the rest two types of adiponectin showed no statistically significant differences.Conclusion The low expression of adiponectin in patients with CHD is located in the EAT and mainly decreased as the form of HMW adiponectin.
作者
夏东升
杜中东
王宏飞
叶霖
袁燕红
汪芹
童路
陈新忠
Xia Dongsheng;Du Zhongdong;Wang Hongfei;Ye Lin;Yuan Yanhong;Wang Qin;Tong Lu;Chen Xinzhong(Department of Cardiovascular Surgery,Fuwai Central China Cardiovascular Hospital,Zhengzhou 450003,China;Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Anesthesiology,Hubei General Hospital of the Chinese People’s Armed Police Forces,Wuhan 430061,China;Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第9期1563-1566,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金面上项目(81270265、81873489)
武汉市应用基础研究项目(2014060101010031).
关键词
脂联素
心外膜脂肪组织
冠心病
Adiponectin
Epicardial adipose tissue
Coronary artery disease
