摘要
为了探究甘蓝型油菜BnaFUS3基因的非生物胁迫表达,以甘蓝型油菜‘湘油15’为供试材料,当油菜长成至6片真叶时期,对其进行6-BA、干旱、高温、低温、胁迫、水渍等逆境胁迫处理。结果表明BnaFUS3的两个拷贝在不同的逆境胁迫处理下其表达也不同。6-BA、干旱和高温处理下BnaA2.FUS3和BnaA6.FUS3的表达量均升高,说明能够诱导和促进BnaFUS3基因的表达,其中6-BA对BnaA6.FUS3的诱导大于BnaA2.FUS3,而在干旱条件下,24 h内BnaA2.FUS3的表达均大于BnaA6.FUS3,说明干旱对BnaA2.FUS3的诱导作用大于BnaA6.FUS3。盐胁迫、低温环境下抑制BnaFUS3的表达;ABA和水渍对BnaFUS3的表达影响波动较大,其中水渍条件下6~12 h内,BnaFUS3表达开始上调,说明此时BnaFUS3表达升高以应对环境;此外,温度的突然大变化,短时间内会抑制BnaFUS3的表达。本研究结果为进一步研究BnaFUS3功能与逆境胁迫的关系提供参考,对油菜品种改良也具有重要意义。
In order to explore the abiotic stress expression of BnaFUS3 gene in Brassica napus,'Xiangyou15' was used as the experimental material. When the rape grows to the 6th leaf stage, it was subjected to stress treatment. The results showed that the expression of two copies of BnaFUS3 was different in different stress conditions. 6-BA, drought and high temperature could induce and promote the expression of BnaFUS3 gene. The induction of BnaA6.FUS3 by 6-BA was greater than BnaA2.FUS3, and the induction of BnaA2.FUS3 was greater than BnaA6.FUS3 in drought. The expression of BnaFUS3 was inhibited under salt stress and low temperature environment. The influence of water logging and ABA on the expression of BnaFUS3 fluctuates. In addition, sudden large changes in temperature will inhibit the expression of BnaFUS3 in a short time. The results of this study provide reference for further study on the relationship between BnaFUS3 function and stress, and are of great significance to the improvement of rape varieties.
作者
张曙
韦云婷
熊腾
王峰
官春云
Zhang Shu;Wei Yunting;Xiong Teng;Wang Feng;Guan Chunyun(National Oilseed Crops Improvement Center Hunan Sub-center,College of Agriculture,Hunan Agricultural University,Changsha,410128)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第16期5209-5214,共6页
Molecular Plant Breeding
基金
国家重点研发计划-中南地区强势油菜杂交中的创制与应用(2016YF010301)
973计划-主要环境因子及生育期影响油菜油脂积累的分子基础(2015CB150206)
湖南省省长专项-双季稻区油菜早熟品种选育和机械化生产技术研究示范专项共同资助
