摘要
利用水酶法处理麻疯树籽得到酶解液中的乳状液及水解液作为复合乳化剂,采用高压均质技术制备麻疯树籽酶解液纳米乳液。研究麻疯树籽酶解液中乳状液和水解液添加量,以及高压均质压力和均质次数对纳米乳液平均粒径、分散性指数(PDI)、ζ-电位、浊度、乳化产率和乳液稳定性指数(TSI)等性质影响,确定纳米乳液最佳制备工艺参数为:乳状液添加量15.55%、水解液添加量45.25%、高压均质压力91MPa、均质次数4次。此条件下,纳米乳液平均粒径为297.2nm,TSI为2.98,乳化产率高达92.47%。通过超高分辨显微镜观测到麻疯树籽酶解液纳米乳液粒径均一、分布均匀,纳米乳液界面蛋白吸附量高达31.20mg/m2。
The emulsion and hydrolysate obtained from enzymatic hydrolysis of Jatropha curcas L. seeds were used as compound emulsifier, and the enzymatic hydrolysate nanoemulsion of Jatropha curcas L. seeds was prepared by high pressure homogenization technology. The effects of additive amount of emulsion and hydrolysate in the enzymatic product of Jatropha curcas L. seeds, as well as high pressure homogenization pressure and frequency on the average particle size, polydispersity index (PDI),ζ-potential, turbidity, emulsion productive rate and TSI of nanoemulsion were studied, results with the highest desirability of preparing the nanoemulsion were obtained with the additive amount of emulsion at15.55%, the additive amount of hydrolysate at 45.25%, high pressure homogenization pressure at 91 MPa and homogenization frequency at four times. Upon these conditions, the average particle size was297.2 nm, turbiscan stability index (TSI) was2.98 and the emulsion productive rate was as high as 92.47%. The enzymatic hydrolysate nanoemulsion of Jatropha curcas L. seeds was finally prepared, it was observed through the super high-resolution microscopy that the particles of it were uniform in the size and evenly distributed in the emulsion system. In addition, it was found that the interfacial protein content of the nanoemulsion was as high as 31.20 mg/m2.
作者
王勇
许晶
连子腾
郭明辉
WANG Yong;XU Jing;LIAN Ziteng;GUO Minghui(Key Laboratory of Bio-based Material Science and Technology, Ministry of Education,Northeast Forestry University, Harbin 150040, China;Office of Graduates Affairs, Northeast Agricultural University, Harbin 150030, China;College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China;College of Food Science, Northeast Agricultural University, Harbin 150030, China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2019年第9期364-372,共9页
Transactions of the Chinese Society for Agricultural Machinery
基金
黑龙江省自然科学基金项目(LC2017010)
关键词
麻疯树籽
乳状液
水解液
高压均质
纳米乳液
稳定性
Jatropha curcas L. seeds
emulsion
hydrolyzate
high pressure homogeneous
nanoemulsion
stability
