摘要
目的观察调脾护心方对慢性心力衰竭大鼠toll样受体4及肿瘤坏死因子-α(TNF-α)表达的影响。方法选择SD雄性健康大鼠40只,随机分为对照组、中药高剂量组、中药低剂量组、西药组和模型组,各8只。将除对照组外的32只大鼠结扎左冠状动脉前降支,建立心肌梗死后心力衰竭大鼠模型。造模成功后,中药高剂量组以23.40mg/(kg·d)标准给予调脾护心方颗粒剂灌胃,中药低剂量组以5.85mg/(kg·d)标准给予调脾护心方颗粒剂灌胃,西药组以0.36mg/(kg·d)标准给予培哚普利灌胃,模型组和对照组给予相同剂量的生理盐水灌胃。连续给药4周后,观察实验期间各组大鼠精神、饮食及饮水、消瘦等一般情况。采用免疫印迹法检测toll样受体4表达水平,酶联免疫吸附实验(ELISA)检测TNF-α表达水平及光镜下各组大鼠心肌细胞变化情况。结果模型组大鼠心肌toll样受体4和TNF-α表达明显高于对照组,差异均有统计学意义(P<0.05);与模型组比较,中药低剂量组和中药高剂量组、西药组心肌toll样受体4和TNF-α表达显著降低,差异均有统计学意义(P<0.05);中药低剂量组与西药组toll样受体4和TNF-α表达比较,差异均有统计学意义(P<0.05)。结论调脾护心方可缓解慢性心力衰竭大鼠炎症状态,减轻临床症状;同时改善心力衰竭炎症状态存在药物剂量浓度差异,在一定范围内,增加调脾护心方剂量,在改善心力衰竭炎症状态方面,达到最佳治疗效果。
Objective To observe the influence of Tiaopi Huxin Formula on the protein expressions of tumor necrosis factor-α(TNF-α),and toll-like receptor 4 in patients with chronic heart failure rats.Methods Forty SD male rats were randomly divided into control group,high-dose group,low-dose group,western medicine group and model group(n=8).The rat model of heart failure after myocardial infarction was established by ligating left anterior descending coronary artery in 32 rats except the control group.After successful modeling,Tiaopi Huxin Formula were gavaged to the high-dose group[23.40 mg/(kg·d)],low-dose group[5.85 mg/(kg·d)],western medicine group was giving perindopril[0.36 mg/(kg·d)],and the model group and the control group were gavaged the same volume of saline.After 4 weeks,the general state of rats′spirit,diet,drinking water and wasting during the experiment was observed,and the expression level of toll like receptor 4 was detected by Western blot,the expression of TNF-αwas detected by ELISA,and the pathological changes of rat myocardial cells in rats were observed after treatment.Results The expression of toll-like receptor 4 and TNF-αin the model group were significantly higher than those in the control group(P<0.05).Compared with the model group,the expressions of toll-like receptor 4 and TNF-αin the low-dose group,high-dose group,and western medicine group were significantly reduced(P<0.05).The expression of toll-like receptor 4 and TNF-αin the low-dose group,and the western medicine group were statistically significant(P<0.05).Conclusion Tiaopi Huxin Formula could relieve inflammation and clinical symptoms in chronic heart failure rats.For improving inflammatory state of heart failure,there are differences in drug dose concentration.Within a certain range,increasing the dose of Tiaopi Huxin Formula could achieve the best therapeutic effect in improving inflammatory state of heart failure.
作者
付军
祝德伟
戴小华
杨帆
王银燕
戚先伟
FU Jun;ZHU Dewei;DAI Xiaohua;YANG Fan;WANG Yinyan;QI Xianwei(The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Institute of Cardiovascular Diseases,Anhui Academy of Traditional Chinese Medicine,Hefei 230031,Anhui,China)
出处
《中西医结合心脑血管病杂志》
2019年第16期2420-2424,共5页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
安徽中医药大学科研项目(No.2016qn007)
