摘要
为提取并利用葵花盘中具有降尿酸和溶解痛风石作用的小分子肽,以小分子肽得率为指标,通过酶水解法优化提取条件,确定碱性蛋白酶为最理想的酶.通过单因素实验考察料液比、时间、温度、pH值和加酶量对小分子肽得率的影响,利用响应面Box-Behnken实验,优化酶水解法提取葵花盘小分子肽的工艺,得到其最佳工艺条件为:加入碱性蛋白酶,m(料)∶V(液)=1∶15,酶的用量为2%,pH=9.0,温度为55℃,时间为2h,葵花盘小分子肽的得率最高为(42.41±0.01)%.
In order to extract and utilize small molecular peptides from the sunflower calathide that could reduce uric acid and dissolve tophi,the extraction conditions were optimized by the enzymatic hydrolysis method with the yield of small molecular peptides as the index,and the alkaline protease was determined to be the most ideal enzyme. The effects of material-to-liquid ratio,time,temperature,pH value and enzyme amount on the yield of small molecular peptides were investigated by single factor test.The response surface Mask-Behnken test was used to optimize the extraction of small molecular peptides from sunflower calathide by enzymatic hydrolysis method.The results show that the optimum technological conditions are adding alkaline protease,m(material)∶V(liquid)=1∶15,the amount of enzyme is 2%,the pH=9.0,the temperature is 55 ℃,and the time is 2 h.The highest yield of small molecular peptides from sunflower calathide is(42.41±0.01)%.
作者
郭宝生
杨翊研
胡峻涵
韩葳葳
李婉南
GUO Baosheng;YANG Yiyan;HU Junhan;HAN Weiwei;LI Wannan(Jilin Institute for Drug Control, Changchun 130033, China;School Hospital of Jilin University, Changchun 130012, China;College of Life Sciences, Jilin University, Changchun 130012, China;Jilin Province Special Medicine Food Biotechnology Co., Ltd., Changchun 130012, China)
出处
《吉林大学学报（理学版）》
CAS
北大核心
2019年第5期1275-1280,共6页
Journal of Jilin University:Science Edition
基金
吉林省自然科学基金(批准号:20170101068JC)
关键词
葵花盘
小分子肽
提取
单因素
酶水解法
响应面法
sunflower calathide
small molecular peptide
extraction
single factor
enzymatic hydrolysis method
response surface method
