摘要
目的研究山楂叶总黄酮对胶质瘤U87细胞的抑制作用。方法体外培养U87细胞,采用25、50、100mg·L^-1的山楂叶总黄酮,通过CCK-8实验、划痕实验、Transwell法、黏附实验,检测山楂叶总黄酮对U87细胞增殖、迁移能力、侵袭能力、黏附能力的影响;克隆形成实验检测细胞克隆形成能力。结果与空白对照组相比,山楂叶总黄酮呈浓度依赖性抑制U87细胞的增殖、迁移、侵袭及黏附能力,差异具有统计学意义(P<0.05),山楂叶总黄酮50mg·L^-1时抑制胶质瘤U87细胞生长作用最为明显。结论山楂叶总黄酮对胶质瘤U87细胞有一定的抑制作用。
Aim To explore the inhibitory effect of hawthorn leaves flavonoids(HLF) on human glioblastoma U87 cells.Methods U87 cells were cultured in vitro.The concentration of HLF was 25,50,100 mg·L^-1 as drug concentration.The effects of HLF on the proliferation,migration,invasion and adhesion of U87 cells were detected by CCK-8 assay,scratch assay,Transwell assay and adhesion assay,and clone forming ability was detected by cell clonogenic assay.Results HLF inhibited the proliferation,migration,invasion and adhesion of U87 cells in a concentration-dependent manner,and the difference was statistically significant compared with control group( P <0.05).When HLF reached 50 mg·L^-1,the inhibition of tumor growth was the most significant.Conclusion HLF has a certain inhibitory effect on glioma U87 cells.
作者
刁婷婷
张雨晨
吕伟
闵清
DIAO Ting-ting;ZHANG Yu-chen;LYU Wei;MIN Qing(School of Pharmacy,Hubei University of Science and Technology,Xianning Hubei 437100,China;Department of pharmacy,The central Hospital of Xianning,Xianning Hubei 437100,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第10期1448-1452,共5页
Chinese Pharmacological Bulletin
基金
湖北省卫生厅中医药研究项目(No 2012Z-B02)
咸宁市科技局科研项目(No 201101)
关键词
山楂叶总黄酮
人胶质母细胞瘤U87细胞
增殖
迁移
侵袭
黏附
hawthorn leaves flavonoids
human glioblastoma U87 cell
proliferation
migration
invasion
adhesion
