摘要
目的探讨七氟醚后处理对失血性休克复苏大鼠海马氧化应激,以及对沉默信息调节因子1(silent information regulation 1,SIRT1)和过氧化物酶体增殖物激活受体γ共激活因子1α(peroxisome proliferator-activated receptor γ coactivator-1α,PGC-1α)表达的影响。方法♂SD大鼠随机分为假手术组(Sham组)、失血性休克复苏组(Shock组)、七氟醚后处理组(Sevo组)。Sevo组经失血性休克后,于血液回输即刻吸入2.4%七氟醚,Sham组和Shock组在相应时间点吸入95%O2,5%CO2混合气体,记录放血即刻(T0)、放血结束即刻(T1)、血液回输即刻(T2)和血液回输结束即刻(T3)的平均动脉压(MAP),并采集动脉血进行血气分析。血液回输结束24h后,检测海马组织丙二醛(MDA)含量和线粒体超氧化物歧化酶(SOD)活性,Western blot测定海马组织中SIRT1和PGC-1α蛋白的表达。结果与Sham组相比,Shock组MDA含量增加,SOD活性降低,SIRT1和PGC-1α蛋白表达上调(P<0.05);与Shock组相比,Sevo组MDA含量减少,SOD活性增强,SIRT1和PGC-1α蛋白表达上调(P<0.05)。结论七氟醚后处理可减轻失血性休克复苏大鼠海马氧化应激,其机制可能与上调SIRT1和PGC-1α的蛋白表达水平有关。
Aim To investigate the effects of sevoflurane postconditioning on oxidative stress and the expression of silent information regulation (SIRT1) and peroxisome proliferator-activated receptor γ coactivator 1α(PGC-1α) in hippocampus of rats subjected to hemorrhagic shock and resuscitation.Methods Male SD rats were randomly divided into sham surgery group (Sham group),shock and resuscitation group (Shock group) and 2.4% sevoflurane postconditioning group (Sevo group).The rats in Sevo group were inhaled 2.4% sevoflurane when received resuscitation after hemorrhagic shock,while rats in Sham and Shock group were treated with 95% O 2 and 5% CO 2 in the corresponding period.MAP and arterial blood gases were measured at T0 (start bleeding),T1 (30 min after bleeding),T2 (start resuscitation),and T3 (30 min after resuscitation).After 24h of surgery,rats with successful model were chosen for the detection of various indexes.The content of malonaldehyde (MDA) in hippocampus and the activity of superoxide dismutase (SOD) in mitochondria isolated from hippocampal tissue were detected.Western blot was used to analyze the protein relative expression levels of SIRT1 and PGC-1α in hippocampus.Results Compared with Sham group,the content of MDA increased,the activity of SOD decreased,and the expression of SIRT1 and PGC-1α increased in Shock group ( P <0.05).Compared with Shock group,the content of MDA decreased,the activity of SOD increased,and the expression of SIRT1 and PGC-1α increased in Sevo group ( P <0.05).Conclusions Sevoflurane postconditioning can alleviate oxidative stress in hippocampus of a model rat of hemorrhagic shock and resuscitation,which may be correlated with the up-regulation of the protein relative expression levels of SIRT1 and PGC-1α.
作者
王妍妍
张丽
黄春霞
朱守峰
杨青青
胡宪文
WANG Yan-yan;ZHAGN Li;HUANG Chun-xia;ZHU Shou-feng;YANG Qing-qing;HU Xian-wen(Dept of Anesthesiology and Perioperative Medicine,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第10期1443-1447,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81471341)
