摘要
在冷鲜滩羊肉贮藏过程中,采用表面增强激光解吸电离飞行时间质谱技术研究其微生物菌体蛋白质,并结合宏基因组测序法分析出微生物群落演替过程中的优势菌,得出二者之间的相关性:随着假单胞菌从最初的9.98%上升到33.70%,热死环丝菌从0%上升到23.98%,与坏死诱导疫霉蛋白质、NADH-泛醌氧化还原酶链4L、生长抑制素-28、ATP合酶F(0)复合体亚基C1、朊蛋白质类呈正相关,与角蛋白质关联蛋白质3-1呈负相关;埃希氏菌从4.35%上升到11.40%,乳酸杆菌从4.02%上升到7.54%,与角蛋白质关联蛋白质8-1、抗菌肽及磷载脂蛋白质C-II呈正相关,与GRF/GHRH生长激素释放素、ATP合成蛋白质、载脂蛋白质E呈负相关;说明随着优势菌的含量增多,呈正相关的菌体蛋白质含量上升且与相对应的优势菌息息相关,而呈负相关的菌体蛋白质会逐渐被优势菌降解,亦导致了优势菌的增长趋势缓慢。
The differential microbial proteins from fresh Tan sheep meat during chilled storage were studied by surface enhanced laser desorption ionization time-of-flight mass spectrometry, and the changes in the dominant bacterial community composition were analyzed by metagenomics. Meanwhile, the correlation between dominant bacteria and differential proteins was examined. The obtained results indicated that the number of Pseudomonas and Brochothrix thermosphacta increased from an initial level of 9.98% and 0% to 33.70% and 23.98% after storage, respectively. They were positively correlated with necrosis-inducing phytophthora protein, MT-ND4L, somatostatin-28, ATP synthase F(0) complex subunit C1 and prion proteins, but negatively correlated with Keratin associated protein 3-1. The number of Escherichia increased from 4.35% to 11.40%, and the number of Lactobacillus increased from 4.02% to 7.54%. They were positively correlated with keratin-associated protein 8-1, antimicrobial peptide and phosphor apolipoprotein C-II, but negatively correlated with growth hormone-releasing hormone, ATP synthase, and apolipoprotein E. An increase in the number of the dominant bacteria corresponded to an increase in the contents of bacterial proteins positively correlated with it, while those negatively correlated with it were gradually degraded, leading to slow growth of the dominant bacteria.
作者
赵晓策
胡倩倩
罗瑞明
张赫宇
ZHAO Xiaoce;HU Qianqian;LUO Ruiming;ZHANG Heyu(College of Agriculture, Ningxia University, Yinchuan 750021, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第18期116-120,共5页
Food Science
基金
国家自然科学基金地区科学基金项目（31460431）
关键词
滩羊肉
菌体蛋白质
蛋白质组学
宏基因组学
优势菌
关联性
Tan sheep meat
differential protein
proteomics
metagenomic
dominant bacteria
correlation
