摘要
目的探讨高效液相色谱串联质谱(HPLC-MS/MS)检测急诊百草枯中毒患者血浆中百草枯水平的可靠性和优越性。方法采用Phenomenex Kinetex mHILIC色谱柱(100.0 mm×2.1 mm,2.6μm),以1%甲酸铵水溶液(含甲酸铵250 mmol/L)和乙腈分别为流动相A、B,建立HPLC-MS/MS(流速0.35 mL/min、检测波长258 nm、柱温40℃),通过受试者工作特征(ROC)曲线评价血浆百草枯水平。结果54.28~13 190.00 ng/mL百草枯的线性关系良好(R 2=0.999 9),批内、批间RSD均<10%。63例急性百草枯中毒患者血浆百草枯水平平均为2 820 ng/mL,死亡组水平显著高于存活组(P<0.05)。结论HPLC-MS/MS能快速、准确、可靠检测急诊百草枯中毒患者血浆的百草枯水平,对患者的治疗具有指导意义。
Objective To investigate the reliability and superiority of high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)in the determination of paraquat concentration in plasma of patients with acute paraquat poisoning.Methods HPLC-MS/MS(flow rate)was established using a Phenomenex Kinetex mHILIC column(100.0 mm×2.1 mm,2.6μm)with 1%aqueous formic acid(containing ammonium formate 250 mmol/L)and acetonitrile as mobile phases A and B,respectively.Plasma paraquat concentration was evaluated by receiver operating characteristic(ROC)curve at 0.35 mL/min,detection wavelength 258 nm,column temperature 40℃.Results The linear relationship of 54.28-13 190.00 ng/mL paraquat was good(R 2=0.999 9),and the intra-assay and inter-assay RSD were all<10%.The plasma concentration of paraquat in 63 patients with acute paraquat poisoning was 2 820 ng/mL,and the death group was significantly higher than the survival group(P<0.05).Conclusion HPLC-MS/MS can quickly,accurately and reliably detect the plasma paraquat concentration in patients with acute paraquat poisoning,which is of guiding significance in the treatment of acute paraquat poisoning patients.To investigate the reliability and superiority of HPLC-MS/MS in the determination of plasma paraquat concentration.
作者
艾娇
樊晶
陈维
AI Jiao;FAN Jing;CHEN Wei(Department of Emergency Medicine,the Fifth People's Hospital of Chengdu,Chengdu,Sichuan 611030,China;Department of Critical Care Medicine,Chengdu Third People's Hospital,Chengdu,Sichuan 611030,China)
出处
《检验医学与临床》
CAS
2019年第17期2478-2481,共4页
Laboratory Medicine and Clinic
关键词
百草枯
高效液相色谱串联质谱法
人血浆
方法学评价
paraquat
high performance liquid chromatography tandem mass spectrometry
human plasma
methodological evaluation
