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HPLC法测定虎黄烧伤搽剂中虎杖苷的含量 被引量:4

Determination the Content of Polydatin in HuHuang Burn Liniment by HPLC
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摘要 目的建立以高效液相色谱法测定虎黄烧伤搽剂中虎杖苷含量的方法。方法采用高效液相色谱法,以虎杖苷为对照品,色谱柱为ZORBAX Eclipse Plus C 18 (250mm×4.6mm,5μm),流动相为乙腈-水(23∶ 77),流速为1.0mL·min^-1 ,柱温为35℃,检测波长为306nm。结果虎杖苷的线性范围为0.10368~0.24192μg(R=0.9998),平均回收率为100.6%(N=6),3批样品含量测定结果均值为:本品每1mL含虎杖苷为0.72mg。结论本方法专属性强,重现性好,且稳定,可靠。 OBJECTIVE To establish the method of determination the content of Polydatin in HuHuang burn liniment by HPLC. METHODS HPLC methods was used and Polydatin was selected as reference substance.The ZORBAX Eclipse plus C 18 column (250mm×4.6mm,5μm) was used.The mobile phase was Acetonitrile-water (23∶ 77),with a flow rate at 1.0mL·min -1 and the column temperature was maintained at 35℃ and wavelength at 306nm. RESULTS The linear range for detection of polydatin was 0.10368~0.24192μg (R=0.9998).The average recovery rate of the method was 100.6%(N=6).The average content of the three batches of samples was 0.72mg per 1mL of polydatin. CONCLUSION The method was reliable and stable with good reproducibility and strong specificity.
作者 陈志平 郑维兵 赵东 钟旭 CHEN Zhi-ping;ZHENG Wei-bing;ZHAO Dong;ZHONG Xu(Chongqing Shengsheng Pharmaceutical Co.,Ltd.,Shizhu 409100,China;Chongqing Xi Xuan Biotechnology Co.,Ltd.,Fuling 408000,China;Chongqing Shouhan Intelligent Technology Research Institute Co.,Ltd.,Fuling 408000,China)
出处 《海峡药学》 2019年第8期110-112,共3页 Strait Pharmaceutical Journal
关键词 虎黄烧伤搽剂 虎杖苷 含量测定 高效液相色谱法 HuHuang burn liniment Polydatin Determination HPLC
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