摘要
目的分析人工建立的铜绿假单胞菌(PA)生物膜对临床常用抗菌药物的耐药情况。方法 利用96孔微量平板建立PA生物膜模型,结晶紫染色法分析PA形成生物膜的能力,纸片扩散法测定浮游态与生物膜态PA的耐药性,微量倍比稀释法测定浮游态PA的最低抑菌浓度,生物膜PA的最小生物膜清除浓度。结果 临床分离的63株PA中有62株(占98.4%)形成生物膜,1株未形成生物膜。生物膜态阿米卡星、哌拉西林/他唑巴坦、替卡西林/克拉维酸、哌拉西林、头孢他啶、头孢哌酮/舒巴坦、头孢哌酮、头孢吡肟、亚胺培南、美罗培南、氨曲南、环丙沙星的PA耐药率均高于浮游态(P<0.01)。生物膜菌最小生物膜清除浓度是浮游菌的10倍以上。结论 通过平板培养法可建立PA生物膜模型,结晶紫染色法半定量得出大部分PA能形成生物膜,生物膜态的PA对临床常用抗菌药物的耐药性高于浮游菌。
Objective To analyze the resistance of artificially established Pseudomonas aeruginosa(PA) biofilm to commonly used antibiotics. Methods The 96 -well microplates were used to establish a model of Pseudomonas aeruginosa biofilm.The ability of PA to form biofilm was analyzed by crystal violet staining.The drug resistance of planktonic and biofilm PA was determined by K-B method.The minimum inhibitory concentration of planktonic PA,and the minimal biofilm eradicate concentration were determined by microdilution method. Results Altoge- ther 62 out of 63 PA strains (98.4%) formed biofilm,while 1 strain did not.The biofilm PA resistance rates to amikacin, piperacillin /tazobactam, ticacillin/clavulanic acid, piperacillin, ceftazidime, cefoperazone/sulbactam, cefoperazone, cefepxime, imipenem, meropenem, aztreonam and ciprofloxacin were all higher than planktonic PA( P <0.01). Minimal biofilm eradicate concentration of biofilm bacteria was 10 times more than that of planktonic bacteria. Conclusion PA biofilm model can be established by plate culture method.Semi-quantitative crystal violet staining shows that most PA can form biofilm.The drug resistance of biofilm PA to commonly used antibiotics in clinic is much higher than that of planktonic bacteria.
作者
刘春都
朱叶飞
LIU Chundu;ZHU Yefei(Medical Examination Center,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,China;Clinical Laboratory,Nanjing Pukou Hospital,Nanjing 210031,China)
出处
《医学综述》
2019年第16期3309-3312,3316,共5页
Medical Recapitulate
关键词
铜绿假单胞菌
生物膜
耐药性
Pseudomonas aeruginosa
Biofilm
Drug resistance
