摘要
为了提高有机硅材料(PDMS)的表面抑菌活性,通过在其表面共价键合富含巯基的二氧化硅微球,并利用巯基的还原性原位生长纳米银,实现在有机硅表面自组装纳米银二氧化硅微球的疏水涂层(WCA=90.33°)。选用大肠杆菌(革兰氏阴性菌)和枯草芽孢杆菌(革兰氏阳性菌)作为测试菌种,通过菌落计数法和活/死细菌染色法研究疏水涂层对细菌的抑制作用。研究结果表明:SiO2@AgNPs与PDMS的复合材料可以有效抑制细菌生长,并不抑制人类细胞的生长。
In order to improve the surface bacteriostatic capacity of PDMS (polydimethylsiloxane), thiol groups-rich silica microspheres were self-assembled on the surface of PDMS by covalent bonds. Subsequently, silver ions were reduced to silver nanoparticles directly by the thiol groups of microspheres(the water contact angle of bacteriostatic coating was about 90.33o). Colony counting and live/dead bacterial staining were used to study the inhibition of bacteria on the surface of this composite material, and E. coli(gram-negative bacteria) and Bacillus subtilis(gram-positive bacteria) were selected as objects. The results show that Ag-SiO2-PDMS composite materials can effectively inhibit the growth of bacteria. Meanwhile, this bacteriostatic coating presents no inhibitory effect on human cells.
作者
沈启慧
单驿轩
连朋亮
刘岩
于东冬
周建光
SHEN Qihui;SHAN Yixuan;LIAN Pengliang;LIU Yan;YU Dongdong;ZHOU Jianguang(Department of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;Hospital of Zhejiang University, Hangzhou 310027, China;State Key Laboratory of Industrial Control Technology, Institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, China)
出处
《中南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2019年第8期1802-1807,共6页
Journal of Central South University:Science and Technology
基金
国家重点研发规划项目(2016YFC0800905-Z03)
国家自然科学基金资助项目(21805107)~~
关键词
复合材料
细菌
抑菌作用
纳米银
composite material
bacteria
bacteriostatic effect
silver nanoparticles
