摘要
目的研究N-甲基-D-天冬氨酸(NMDA)对原代培养的大鼠颅盖骨成骨细胞[Ca^2+]i水平的影响。方法利用钙离子成像系统检测不同浓度NMDA(100~500 μmol/L)对原代培养的大鼠颅盖骨成骨细胞[Ca^2+]i水平的影响和NMDA受体非竞争性拮抗剂MK801(Dizocilpin,地佐西平)干预后的细胞[Ca^2+]i水平。结果不同浓度的NMDA导致不同程度和不同方式的细胞内[Ca^2+]i增加,各浓度NMDA引起成骨细胞[Ca2+]i一过性增加之后还可引起继发性变化(钙震荡或缓慢持续升高)。MK801不同程度地抑制NMDA诱导的[Ca^2+]i增加。结论原代培养的大鼠颅盖骨成骨细胞表达丰富的功能性NMDA受体,并且对不同浓度的NMDA呈不同方式和程度的[Ca^2+]i增加。MK-801对其不完全阻断的作用特点,提示原代培养的大鼠颅盖骨成骨细胞的NMDA受体与中枢神经系统相比其通道性质有所不同。
Objective To study the effect of N-Methyl-D-asparticacid (NMDA) on the intracellular free calcium concentration ([Ca^2+]i) in primary cultured rat calvaria osteoblasts. Methods A calcium imaging technique was applied to observe [Ca^2+]i changes in primary cultured rat calvaria osteoblasts after stimulating by NMDA with various concentrations or pretreated with NMDA receptor noncompetitive antagonism MK801 (Dizocilpin). Results Different concentrations of NMDA caused [Ca^2+]i increases in varying degrees and by different ways. NMDA could evoke transient increase and secondary change in [Ca^2+]i including calcium oscillation or steady increase. MK801 inhibited NMDA-induced [Ca^2+]i increase in varying degrees. Conclusion These results indicated that there are abundant functional NMDA receptors expressed in primary cultured rat calvaria osteoblasts, showing different forms and varying degrees of [Ca^2+]i increases in response to different concentrations of NMDA. The characters of the blocking effect of MK801 to NMDA-induced [Ca^2+]i increasing, indicated that the NMDA receptors expressed in primary cultured rat calvaria osteoblasts differ in channel properties from those in central nervous system.
作者
刘燕卿
赵琳
李胜天
刘建民
Liu Yanqing;Zhao Lin;Li Shengtian;Liu Jianmin(Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China;Department of Endocrinology, Shanghai Putuo District People′s Hospital, Shanghai 200060, China)
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2019年第8期703-710,共8页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金(30570881).
