摘要
目的了解海南类鼻疽伯克霍尔德菌(Bp)致病株的病原学及多位点序列分型(MLST)特征,为研究海南致病性Bp的菌群结构特征和系统发育特征提供参考依据.方法收集2011-2018年在海南省人民医院就诊的海南类鼻疽患者病例资料,以及自患者感染标本中分离培养的Bp致病株.经MLST法对Bp致病株进行基因分型,分析其序列型(ST)地区分布特点;应用eBURSTv3软件对所有Bp致病株的ST进行系统发育分析,建立进化关系图谱.结果共收集91例类鼻疽患者病例资料,其中男性76例、女性15例;年龄主要集中在40~70岁;临床表现包括败血症、肺部感染及局部脓肿;患者基础疾病以糖尿病最为多见.共观察Bp致病株91株,其中有85株Bp致病株分布在海南沿海地区,占93.41%;经MIST法鉴定,共包括39种ST,最常见的为ST46(13株)、ST55(12株)、ST50(8株)和ST58(7株),分别占14.29%、13.19%、8.79%和7.69%;除ST46广泛分布在海南沿海地区外,ST55、ST50和ST58分别集中分布在海南的西南、东北和东南部地区;与MLST数据库对比发现,ST30(3株)目前仅在海南发现,ST562(4株)在澳大利亚北部曾有报道,其余ST皆在亚洲东南部地区曾有报道.eBURSTv3软件将39种ST分为3个分型组和18个单个型,其中,以ST300为起源型的分型组中ST最多,包括17种ST、57株Bp致病株;与MLST数据库对比发现,ST300主要分布在泰国等东南亚地区.结论海南Bp致病株的ST具有较高的地区差异性和遗传多样性,且与泰国等东南亚地区的Bp亲缘关系相近.
Objective To understand the pathogenicity and multilocus sequence typing (MLST) characteristics of the pathogenic strain of Burkholderia pseudomallei (Bp) in Hainan, and provide a reference for studying the structural characteristics and phylogenetic characteristics of pathogenic Bp in Hainan. Methods The data of patients with melioidosis in Hainan, who were treated in Hainan General Hospital from 2011 to 2018, and Bp pathogenic strains isolated from patient infected specimens were collected. The Bp pathogenic strains were genotyped by MLST method, and the distribution characteristics of the sequence type (ST) were analyzed. The ST of all Bp pathogenic strains was analyzed by eBURST v3 software to establish the evolutionary relationship map. Results A total of 91 cases of melioidosis patients were studied, including 76 males and 15 females;the ages were mainly concentrated in 40 - 70 years old;clinical manifestations included sepsis, pulmonary infection and local abscess;and the most common diseases were diabetes. A total of 91 Bp pathogenic strains were observed, of which 85 Bp pathogenic strains were distributed in coastal areas of Hainan, accounting for 93.41%;identified by MLST method, 39 ST were discovered, the most common were ST46 (13 strains), ST55 (12 strains), ST50 (8 strains) and ST58 (7 strains), accounting for 14.29%, 13.19%, 8.79% and 7.69%, respectively;except ST46 was widely distributed in the coastal areas of Hainan, the ST55, ST50 and ST58 were concentrated in the southwest, northeast and southeast regions of Hainan. Compared with the MLST database, ST30 (3 strains) was currently found only in Hainan, ST562 (4 strains) had been reported in northern Australia, and the remaining ST models had been reported in southeast Asia. The eBURST v3 software divided the 39 ST into 3 subtypes and 18 individual types. Among them, the subtypes with ST300 as the original type had the most number of ST, including 17 ST, 57 Bp pathogenic strains;compared with the MLST database, the ST300 was mainly distributed in
作者
刘玲丽
王旭明
郑霄
吴华
周晓君
郭红荔
徐凯
李天娇
Liu Lingli;Wang Xuming;Zheng Xiao;Wu Hua;Zhou Xiaojun;Guo Hongli;Xu Kai;Li Tianjiao(Department of Clinical Laboratory,Key Laboratory of Cellular and Molecular Genetic Transformation Medicine,Hainan General Hospital,Haikou 570311,China;State Key Laboratory for Infectious Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2019年第8期633-637,共5页
Chinese Journal of Endemiology
基金
海南省自然科学基金(817319、818QN310).
