摘要
目的:观察电针五脏俞对失眠大鼠睡眠时间及脑内神经递质、细胞因子水平的影响,探讨电针五脏俞治疗失眠的机制。方法:SD大鼠随机分为对照组、模型组、电针组和地西泮组,每组10只。采用氯苯丙氨酸诱导构建失眠大鼠模型。电针组予以电针"肺俞""心俞""肝俞""脾俞""肾俞",频率60 Hz,强度1 mA,10 min/次,西药组予以地西泮灌胃治疗(0.92 mg/kg),均每日1次,共治疗6 d。评估各组大鼠睡眠持续时间变化,HE染色检测治疗后大鼠海马病理学改变,ELISA法检测下丘脑5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β含量,免疫组织化学法、荧光定量PCR法和Western blot法检测海马组织外周型苯二氮卓受体(PBR)mRNA及蛋白表达。结果:与对照组比较,模型组大鼠昼夜节律紊乱,睡眠持续时间显著缩短、体质量下降(P<0.05);与模型组比较,电针组及地西泮组大鼠睡眠持续时间增长、体质量增加(P<0.05)。与对照组比较,模型组大鼠海马组织神经元数量减少,细胞排列不规则,下丘脑5-HT、5-HIAA、TNF-α和IL-1β表达水平均显著降低(P<0.05),海马组织中PBR mRNA和蛋白表达水平显著升高(P<0.05)。与模型组比较,电针组和地西泮组海马组织神经元数量增加,细胞排列逐渐规则,下丘脑中5-HT、5-HIAA、TNF-α和IL-1β表达水平均显著升高(P<0.05),海马组织中PBR mRNA和蛋白表达水平显著降低(P<0.05)。电针在增加睡眠持续时间、体质量、下丘脑5-HT和5-HIAA表达方面与地西泮疗效相当,在升高下丘脑TNF-α和IL-1β表达水平、降低海马组织PBR mRNA及蛋白表达方面优于地西泮(P<0.05)。结论:电针五脏俞能够降低海马组织中PBR表达,促进下丘脑神经递质及细胞因子的合成和释放,缓解海马组织的病理损伤,改善失眠大鼠的症状。
Objective To observe the therapeutic effect of electroacupuncture(EA)at five Back-Shu points on sleep,hippocampal peripheral benzodiazepine receptor(PBR) expression and hypothalamic 5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA),tumor necrosis factor alpha(TNF-α)and interleukin(IL)-1β contents in insomnia rats,so as to explore its mechanisms underlying improvement of insomnia.Methods Forty male SD rats were randomly divided into control,model,EA and medication(Diazepam)groups(n=10 rats in each group).The insomnia model was established by intraperitoneal injection(i.p.)of para-Chlorophenylalanine(PCPA,300 mg/kg)once daily for2 days.EA(60 Hz,1 mA)was applied to bilateral five Back-Shu points,i.e.,Feishu(BL13),Xinshu(BL15),Ganshu(BL18),Pishu(BL20)and Shenshu(BL23)for 10 min,once daily for 6 days.Rats of the medication group were treated by gavage of Diazepam(0.92 mg/kg)once daily for 6 days.The sleep duration was recorded after i.p.of Pentobarbital Sodium(45 mg/kg).Histopathological changes of the hippocampus were displayed by H.E.staining.The contents of 5-HT,5-HIAA,TNF-α and IL-1β in the hypothalamus were assessed by using ELISA.The expression levels of PBR mRNA and protein in the hippocampus were detected by quantitative real-time PCR,immunohistochemistry and Western blot,separately.Results Following modeling,the sleep duration was considerably shortened in rats of the model group relevant to the control group(P<0.05).After the treatment,the sleep duration was significantly increased in both EA and medication groups relevant to the model group(P<0.05).After modeling,the hippocampal neurons were obviously decreased in number,and disordered in the arrangement,the levels of hypothalamic 5-HT,5-HIAA,TNF-α,and IL-1β were significantly down-regulated(P<0.05),and the expression of PBR mRNA and protein in hippocampus was obviously increased relevant to the control group(P<0.05).Following the treatment,the hippocampal neurons were increased in number and arranged regularly,and the contents of hypothala
作者
唐雷
游菲
胡星
李云飞
TANG Lei;YOU Fei;HU Xing;LI Yun-fei(Department of Rehabilitation,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2019年第8期560-565,共6页
Acupuncture Research
基金
武汉市卫生局资助项目（No.WZ16A03）
国家自然科学基金项目（No.81603699）
关键词
电针
五脏俞
失眠
外周型苯二氮卓受体
Electroacupuncture
Five Back-Shu points
Insomnia
Peripheral benzodiazepine receptor
