摘要
目的探讨黄芪甲苷(AS-Ⅳ)对人内皮祖细胞(EPCs)活性及体外增殖功能的影响,为进一步研究AS-Ⅳ作用于内皮祖细胞介导的血管新生奠定基础。方法从足月健康新生儿脐带血中采用密度梯度离心法分离出单个核细胞,经传代培养,当细胞呈现梭形时对细胞进行鉴定,得到EPCs。将获得的EPCs随机分为实验组和对照组,实验组用不同浓度梯度(25、50、100、200、400 mg/L)的AS-Ⅳ与EPCs进行联合培养,对照组用等量的PBS液处理。通过CCK-8细胞增殖实验研究AS-Ⅳ对内皮祖细胞增殖的影响,及在其促EPCs增殖的最佳浓度下测量EPCs细胞活率。结果经CD31抗体和4’,6-二脒基-2-苯基吲哚(DAPI)核染检测证实成功获取EPCs。AS-Ⅳ能促进EPCs的增殖,其发挥促EPCs增殖的最佳浓度为100 mg/L。AS-Ⅳ干预后内皮祖细胞活率为98.70%,高于对照组的98.12%,差异有统计学意义(χ^2=49.59,P<0.01)。结论黄芪甲苷能增强人EPCs的活性和促进其体外增殖。
Objective The objective is to probe into the effects of astragaloside Ⅳ(AS-Ⅳ) on activity and proliferative function of endothelial progenitor cells (EPCs),which lays a foundation for further study on the effects of AS-Ⅳ on vascular neovascularization mediated by endothelial progenitor cells.Methods The mononuclear cells were isolated by the density gradient centrifugation in umbilical cord blood of full-term healthy infants,and EPCs were obtained by subculture and cell identification when the cells presented spindle shapes.The obtained EPCs were randomly divided into the experimental group and the control group.In the experimental group,EPCs were cultured by AS-Ⅳ with different concentration gradients (25 mg/L,50 mg/L,100 mg/L,200 mg/L and 400 mg/L),while in the control group,they were treated with the same amount of phosphate buffer saline (PBS) solutions.The effects of AS-Ⅳ on the proliferation of endothelial progenitor cells was studied by cell counting kit-8 (CCK-8) cell proliferation experiment,and the activity rate of EPCs cells was measured at the optimum concentration of EPCs proliferation.Results EPCs were successfully obtained after confirming nuclear staining test of CD31 antibody and 4′,6-diamidino-2-phenylindole (DAPI).Further study showed that AS-Ⅳ can promote the proliferation of EPCs,and its optimal concentration of EPCs proliferation is 100 mg/L.Compared with the normal control group,the activity rate of endothelial progenitor cells after intervention of AS-Ⅳ was 98.7%,higher than 98.12% in the control group,with significant difference (χ^2=49.59,P<0.01).Conclusions AS-Ⅳ can enhance the activity of human EPCs and promote their proliferation in vitro.
作者
熊武
陈四红
王禹萌
杨双喜
余亦程
皇毅
王婷婷
张红莲
Xiong Wu;Chen Sihong;Wang Yumeng;Yang Shuangxi;Yu Yicheng;Huang Yi;Wang Tingting;Zhang Honglian(Department of Burn and Plastic Surgery,the First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Department of Pre-treatment Center,the First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;School of Postgraduate,Hunan University of Chinese Medicine,Changsha 410208,China;Department of Plastic Surgery,the 3rd Xiangya Hospital of Central South University,Changsha 410013,China)
出处
《中国医师杂志》
CAS
2019年第8期1152-1155,共4页
Journal of Chinese Physician
基金
湖南省卫生计生委科研计划课题项目(B20180739)~~
关键词
黄芪
皂苷类
内皮祖细胞
细胞增殖
细胞存活
体外研究
Astragalus membranaceus
Saponins
Endothelial progenitor cells
Cell proliferation
Cell survival
In vitro
