摘要
目的探讨载淫羊藿苷/凹凸棒石/Ⅰ型胶原/聚己内酯(icariin/attapulgite/collagen typeⅠ/polycaprolactone,ICA/ATP/ColⅠ/PCL)复合支架修复兔胫骨缺损的效果。方法利用溶液铸浇-粒子滤沥法分别构建ICA/20%ATP/ColⅠ/PCL(支架1)、ICA/30%ATP/ColⅠ/PCL(支架2)、20%ATP/ColⅠ/PCL(支架3)、30%ATP/ColⅠ/PCL(支架4)复合支架材料,采用扫描电镜观察支架2交联前、后结构特征,测量支架2、4的表面接触角检测材料吸水性能,采用体外降解实验评价支架2的药物缓释效果。取30只雄性日本大耳白兔,体质量(2.0±0.1)kg,随机分为A、B、C、D、E 5组,每组6只;制备直径1 cm双侧胫骨缺损模型后,A组不植入任何材料,B^E组分别于骨缺损处植入支架3、支架4、支架1、支架2。术后4、8、12周大体观察骨缺损修复效果;行HE、Masson染色以及成骨特异性转录因子RUNX2、成骨相关转录因子Osterix(OSX)、ColⅠ、骨桥蛋白(osteopontin,OPN)抗体的免疫组织化学染色,观察不同支架材料修复骨缺损效果。结果扫描电镜观察示,支架2为多孔结构,交联前结构疏松,交联后结构致密;表面接触角检测示支架为疏水性材料,且支架2疏水性比支架4更高;药物体外缓释效果显示支架2上药物能微量长效释放。动物体内植入实验中,大体观察示术后4、12周D、E组缺损明显小于A、B、C组。HE和Masson染色示,术后4周A组缺损区域充满大量结缔组织,B、C组可见大量纤维组织,D、E组可见少量新生骨;术后8周各组新生骨比4周时增加;术后12周A组缺损区域仍以纤维组织为主,B、C组可见少量新生骨组织,D、E组可见大量新生骨组织,尤以E组明显,且大部分支架降解。免疫组织化学染色示,术后4周,D、E组新生组织中RUNX2和OSX表达明显高于其余各组,术后8、12周RUNX2表达与4周相比表达下降;术后8、12周与4周相比,各组ColⅠ、OPN表达均增加,且D、E组新生组织中ColⅠ、OPN表达明显高于其�
Objective To investigate the effect of icarin/attapulgite/collagen type Ⅰ/polycaprolactone(ICA/ATP/Col Ⅰ/PCL) composite scaffold in repair of rabbit tibia defect. Methods The ICA/20%ATP/Col Ⅰ/PCL(scaffold 1), ICA/30%ATP/Col Ⅰ/PCL(scaffold 2), 20%ATP/Col Ⅰ/PCL(scaffold 3), and 30%ATP/Col Ⅰ/PCL(scaffold4) composite scaffolds were constructed by solution casting-particle filtration method. The structure characteristics of the scaffold 2 before and after cross-linking were observed by scanning electron microscopy, and the surface contact angles of the scaffold 2 and the scaffold 4 were used to evaluate the water absorption performance of the material. The in vitro degradation test was used to evaluate the sustained-release effect of the scaffold 2. Thirty male Japanese white rabbits,weighing(2.0±0.1) kg, were randomly divided into groups A, B, C, D, and E, 6 in each group. After making a 1 cm-diameter bilateral tibial defects model, group A was the defect control group without any material implanted. Groups B, C,D, and E were implanted with scaffolds 3, 4, 1, and 2 at the defect sites, respectively. At 4, 8, and 12 weeks after operation,the repairing effects of 4 scaffolds were observed by gross observation, histological observation of HE and Masson staining,and immunohistochemical staining of osteogenic specific transcription factor(runt-related transcription factor 2,RUNX2), osteogenic related transcription factor [Osterix(OSX), Col Ⅰ, osteopontin(OPN)]. Results Scanning electron microscopy observation showed that the scaffolds were all porous. The structure of the material was loose before and after cross-linking. The surface contact angle showed that the scaffold was hydrophobic, and the scaffold 2 was more hydrophobic than scaffold 4. The sustained-release effect in vitro showed that the drug could be released in a micro and long-term manner. In the animal implantation experiment, the gross observation showed that the defects were significantly smaller in groups D and E than in groups A, B, and C
作者
宁钰
秦文
任亚辉
李辰凯
陈文扬
赵红斌
NING Yu;QIN Wen;REN Yahui;LI Chenkai;CHEN Wenyang;ZHAO Hongbin(School of Basic Medical Sciences,Gansu University of Traditional Chinese Medicine,Lanzhou Gansu,730000,P.R.China;Department of Orthopaedic Laboratory,Changzhou Second People's Hospital,Changzhou Jiangsu,213000,P.R.China)
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2019年第9期1181-1189,共9页
Chinese Journal of Reparative and Reconstructive Surgery
基金
江苏省科技重点研发项目(BE2018644)~~
关键词
淫羊藿苷
凹凸棒石
Ⅰ型胶原
聚己内酯
复合支架
组织工程骨
Icariin
attapulgite
collagen type Ⅰ
polycaprolactone
composite scaffold
tissue engineered bone
