摘要
随着科学技术的进步,荧光碳质材料越来越多受到人们的关注。具有高荧光量子产率碳点的研制对其在荧光传感等应用具有重要意义。本文以柠檬酸铵和三乙基四胺为原料,采用一步微波法制备了一种低成本、绿色环保、荧光量子产率高(29.83%)的水溶性荧光碳点(N-CDs),并对N-CDs的结构和光学性能进行了表征。N-CDs具有高荧光、良好的生物相容性和光学稳定性。与其它已报道的碳点纳米材料相比,N-CDs具有更加突出的光学性质以及对Cu^2+高选择、高敏感的检测,即合成的N-CDs的荧光强度能够被Cu^2+猝灭且在0.01~11μM存在良好的线性,对Cu2+的检出限为4.5nM(S/N=3),进一步应用到实际水样中Cu2+含量的测定。此外,合成的N-CDs还可用于绘制荧光图案。最后,将强发光、低毒的N-CDs应用于活细胞(SMMC-7721)的荧光图像和细胞中监测Cu^2+的存在,表明合成的N-CDs具有广阔的应用前景。
Water-soluble luminescent nitrogen-doped CDs (N-CDs) having a high QY of 29.83% were prepared by a one-step microwave-assisted pyrolysis method using ammonium citrate and triethylenetetramine as the precursors. The microstructure, optical properties and biocompatibility of the N-CDs were investigated. Results indicate that they exhibit a strong fluorescence, favorable biocompatibility and excellent optical stability. Their photoluminescence is quenched by Cu^2+ ions through the formation of a complex between the N-CDs and Cu^2+, which can be used for Cu^2+ detection. Compared with other CD-based nanomaterials, the N-CDs show outstanding optical properties, and excellent selectivity and sensitivity for Cu^2+ detection with a linear range of 0.01-11 μ M and a detection limit of 4.5 nM. The selectivity for Cu^2+ detection is so good that the N-CDs have been successfully used in Cu^2+ detection for real water samples. They have also been used to draw fluorescent patterns and image living cells.
作者
刘文
张荣
康玉
张晓瑛
王浩江
李丽红
刁海鹏
魏文珑
LIU Wen;ZHANG Rong;KANG Yu;ZHANG Xiao-ying;WANG Hao-jiang;LI Li-hong;DIAO Hai-peng;WEI Wen-long(School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, China;School of Shanxi provincial Traditional Chinese medicine, Taiyuan 030012, China;Department of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China)
出处
《新型炭材料》
SCIE
EI
CAS
CSCD
北大核心
2019年第4期390-402,共13页
New Carbon Materials
基金
National Natural Science Foundation of China(21705104)
Key Research and Development Projects of Shanxi Province(201703D321015-2)
Youth Science Foundation of Shanxi Province(201701D221064)
Science&Technology Innovation Fund of Shanxi Medical University(C01201003),Ph.D Startup Fund of Shanxi Medical University(BS201723)~~
关键词
碳点
高荧光量子产率
Cu^2+传感
荧光绘画
细胞成像
Carbon dots
Highly fluorescence quantum yield
Cu^2+ sensor
Fluorescent patterns
Cells imaging
