摘要
从牛舌鳞状上皮细胞和股骨骨髓细胞中分别克隆出牛β-防御素LAP和牛CATHL2抗菌肽,然后利用重叠延伸PCR克隆得到重组牛LAP-CATHL2抗菌肽,将其克隆进pGEX-4T-1载体,构建了重组牛LAP-CATHL2抗菌肽的原核表达载体,并转进感受态大肠杆菌BL21进行诱导表达,并加以纯化,得到了重组牛LAP-CATHL2抗菌肽产量是2.83 mg·mL-1培养液。
In this study, bovine beta-defensin LAP and bovine CATHL2 antibiotics peptide were cloned from bovine squamous epithelial cells and femoral bone marrow cells, respectively. And then, recombinant bovine LAP-CATHL2 antibiotics peptide were obtained by overlapping extended PCR cloning. Then, recombinant cattle LAP-CATHL2 antibiotics peptide were cloned into pGEX- 4T-1 vector. And the prokaryotic expression vector of recombinant bovine LAP-CATHL2 antibiotics peptide were constructed. The yield of recombinant bovine LAP-CATHL2 antibiotics peptide in the prokaryotic expression vector is 2.83 mg·mL^-1 cultures.
作者
王静
高利
Wang Jing;Gao Li(Northeast Agricultural University,Harbin 150030;Heilongjiang Agriculture Economics Vocational College)
出处
《黑龙江八一农垦大学学报》
2019年第4期40-49,共10页
journal of heilongjiang bayi agricultural university
基金
“十三五”国家重点研发项目(2017YFD0502202）
