摘要
为了解中山病在我国的流行和分布情况,本研究采用竞争ELISA检测方法,对2016-2018年我国吉林、辽宁、内蒙古、河北、新疆、西藏、湖北、重庆、四川、贵州、云南、广西和广东13个省级区划的8 232份牛羊血清进行检测。结果显示,有12个省级区划检测出抗体阳性,且阳性率有明显的地域性和季节性,由北向南逐渐升高(0~74.03%),秋季阳性率(2.50%~60.00%)明显高于春季(0~10.00%)和夏季(2.22%~35.00%)。乌兰察布、哈密、河池和曲靖等4个采样点的牛羊血清检测发现,牛血清抗体阳性率均高于山羊和绵羊血清。以上结果表明中山病在我国的流行已具有广泛性,流行分布状况与其媒介昆虫的活动有密切关系,并且牛比羊更易被该病毒感染,同时本研究的试验数据将有助于我国更好地了解和防控中山病。
To investigate the epidemic distribution of Chuzan disease in China,a total of 8 232 cattle,goat and sheep sera derived from 13 provinces in China between 2016 and 2018 were detected for the antibodies against Chuzan virus(CHUV)by C-ELISA test.The results showed that the CHUV antibodies were observed in 12 provinces,the positive rate of CHUV increases from north to south(0-74.03%),and the infection rate in autumn(September to November)was the highest(2.50%-60.00%),which was significantly higher than that in spring(0-10.00%)and summer(2.22%-35.00%).Detection of sera from cattle,goat sand sheep in Ulanchab,Hami,Hechi and Qujing were conducted,the results showed that the positive rate of cattle sera was higher than that of goat and sheep serum.On the one hand our findings showed that the Chuzan disease was prevalent and provide the reliable reference regarding CHUV surveillance in China,on the other hand it was verified that the prevalence of CHUV was closely related to the activities of its media insects and has obvious regional and seasonal characteristics.
作者
杨振兴
朱沛
李占鸿
廖德芳
朱建波
肖雷
谢佳芮
李华春
杨恒
YANG Zhenxing;ZHU Pei;LI Zhanhong;LIAO Defang;ZHU Jianbo;XIAO Lei;XIE Jiarui;LI Huachun;YANG Heng(Yunnan Tropical and Subtropical Animal Virus Disease Laboratory,YunnanVeterinary and Animal Science Institute,Kunming 650224,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第8期1715-1722,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
基础研究计划青年项目(2016FD002)
国家重点研发计划(2016YFD0500908
2017YFC1200505)
关键词
中山病
血清学调查
竞争ELISA
阳性率
Chuzan disease
serological investigation
competitive ELISA(C-ELISA)
seroprevalence
