摘要
旨在揭示鹅成熟卵泡壁层中各个结构组分的形态特点与发育规律,为禽类成熟卵泡动态发育的分级提供新的视角与依据。本研究选用健康的高产期天府肉鹅母鹅5只,取其各个阶段(直径0~2、2~4、4~6、6~8、8~10 mm与F5~F1)的成熟卵泡进行固定。在0~10 mm中的每个阶段分别随机选取至少3个卵泡,其中每个卵泡(除0~2 mm阶段)均随机选取3个以上不同区域进行HE切片制作,而F5~F1阶段的每个卵泡则随机选取3个以上不同区域进行HE切片制作。每个切片随机取3个以上的区域进行图像采集,分别在每张图像上随机选取3个区域对其对应的卵泡壁层、颗粒层、膜层与结缔层的组织厚度进行测量、统计与分析,对其形态结构进行比较与观察。结果表明:1)在部分直径2 mm以下的卵泡中,还未形成完整的内膜层。2)直径2~10 mm的卵泡与F5卵泡在卵泡尺寸与卵泡壁层形态上并未表现出明显的差异。3)F4~F2阶段的卵泡中,其颗粒层细胞呈扁平状;而膜层与结缔层厚度显著增加(P<0.05),卵泡壁层整体形态发生了较大的改变。4)F1阶段卵泡的颗粒层为疏松的立方状,且其卵泡壁的各个组分厚度也均出现了显著的变化(P<0.05)。结果提示,从成熟卵泡壁层组织形态和结构分析,鹅卵泡的发育历程可划分为2 mm以下、2~10 mm、F5、F4~F2与F1卵泡5个不同的阶段,这些阶段均有其独特的形态、结构特点,且这样的特点很可能与其功能的差异密切相关。
The purpose of this study was to reveal the morphological characteristics and developmental regularity of various components in the mature follicle wall layer of goose,and to provide a new perspective for the grading of dynamic development of mature follicles in poultry.The 5 healthy female geese from maternal line of Tianfu geese in high-yielding period were selected,and their mature follicles at each stage(diameter 0-2,2-4,4-6,6-8,8-10 mm and F5-F1)were fixed.At least 3 follicles were randomly selected in each stage during 0-10 mm,and more than 3 different regions were randomly selected for HE slicing in each follicle(except 0-2 mm stage);While more than 3 different regions were randomly selected for HE slicing in each follicle during F5-F1 stage.More than 3 areas were randomly selected from each slice for image acquisition,and 3 areas were randomly selected from each image to measure.We counted and analyzed the thickness of follicular wall,granulosa layer,theca layer and connective layer,and observed and compared their morphological characteristics.The results showed that:1)There was no complete internal theca layer in some follicles less than 2 mm in diameter.2)There was no significant difference in follicle size and follicular wall morphology between 2-10 mm and F5 follicles.3)In stage F4-F2 follicles,the granulosa layer cells were flat,while the thickness of theca and connective layer increased significantly(P<0.05),and the morphology of follicular wall layer changed greatly.4)The granulosa layer of F1 follicle became loose cubes,and the thickness of each component of follicular wall changed significantly(P<0.05).These results indicate that the development process of goose mature follicle can be divided into 5 stages:0-2 mm,2-10 mm,F5,F4-F2 and F1.These follicles at different stages have unique morphological and structural characteristics,which may be closely related to their functional differences.
作者
甘翔
王继文
李琴
邓艳
胡继伟
李亮
韩春春
GAN Xiang;WANG Jiwen;LI Qin;DENG Yan;HU Jiwei;LI Liang;HAN Chunchun(Animal Genetic Resources Exploration and Innovation Key Laboratory of SichuanProvince,Sichuan Agricultural University,Chengdu 611130,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第8期1607-1613,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31672424)
关键词
鹅
成熟卵泡
卵泡壁层
发育
分级
goose
mature follicle
follicular wall
development
grading
