摘要
目的比较盐酸戊乙奎醚(PHC)及右美托咪定(DEX)对内毒素脂多糖(LPS)致大鼠急性肺损伤(ALI)的保护性作用。方法40只成年SD大鼠,按照随机数字表法分为4组(n=10):0.9%生理盐水对照组(对照组)、LPS致ALI模型组(LPS组)、DEX治疗组(L+D组)、PHC治疗组(L+P组)。除对照组外,实验组采用LPS建立大鼠ALI模型。治疗组在LPS注射后静脉注射DEX或PHC,随后泵入,直到实验结束。对照组注入等量0.9%生理盐水。测定血浆肿瘤坏死因子(TNF-α)、白细胞介素(IL)-1β及IL-6水平;计算肺湿/干重(W/D)比值;取右肺上叶,进行弥漫性肺泡损伤标准(DAD)评分;左肺支气管灌洗,采集肺泡灌洗液(BALF),测定TNF-α、IL-6、总蛋白(TP)水平。结果与对照组比较,ALI组、L+D组、L+P组炎性因子、W/D比值、DAD评分及BALF中TP均升高(P<0.05);与ALI组比较,L+D组、L+P组上述各项观察指标均降低(P<0.05);L+P组与L+D组各项观察指标比较,差异无统计学意义(P>0.05)。结论PHC和DEX均可减轻LPS致大鼠ALI。
Objective To compare the protective effects of pentyl hydrochloride(PHC)and dexmedetomidine(DEX)on acute lung injury(ALI)induced by endotoxin lipopolysaccharide(LPS)in rats.Methods A total of 40 adult SD rats were randomly divided into four groups(n=10)according to the random number table:the 0.9%saline control group(control group),the LPS induced ALI model group(LPS group),the DEX treatment group(L+D Group)and the PHC treatment group(L+P group).Except for the control group,ALI rats models were established with the way of administering intravenous injections of LPS in all experimental groups.The plasma tumor necrosis factorα(TNF-α),interleukin(IL)-1βand IL-6 levels were measured,the lung wet/dry weight(W/D)ratio was calculated,the right lung upper lobe was taken for evaluating diffuse alveolar damage standard(DAD)score,and left lung bronchial lavage bronchoalveolar lavage fluid(BALF)was collected to determine the levels of TNF-α,IL-6 and total protein(TP).Results Compared with the control group,the inflammatory factors levels,W/D ratio,DAD score and TP level in BALF were increased in the ALI group,the L+D group and the L+P group(P<0.05).Compared with the ALI group,all observation indexes mentioned above in the L+D group and the L+P group were decreased(P<0.05).There was no significant difference in all observation indexes between the L+P group and the L+D group(P>0.05).Conclusion The PHC and DEX both can alleviate LPS-induced ALI in rats.
作者
孟志鹏
刘静
胡四平
童飞
龚浩杰
MENG Zhipeng;LIU Jing;HU Siping;TONG Fei;GONG Haojie(Department of Anesthesiology,the Central Hospital of Huzhou,Huzhou,Zhejiang 313000,China;Department of Anesthesiology,the Huzhou Maternal and Child Health Hospital,Huzhou,Zhejiang 313000,China)
出处
《重庆医学》
CAS
2019年第15期2541-2544,共4页
Chongqing medicine
基金
浙江省湖州市重点公益类项目(2015GZ17)
