摘要
红白花鲫胚胎发育期先观察到黑色素细胞的发生,孵化出膜后先后出现黄色素细胞、红色素细胞及虹彩细胞。1月龄花鲫幼苗体色呈浅青灰色,2月龄前后,花鲫皮肤黑色素细胞逐渐减少,体色发生“青转花”的变化,3月龄时基本形成红白镶嵌的成体体色。克隆获得了鲫Slc7a11和Pnp4a体色基因的全长cDNA。鲫Slc7a11全长1782bp,编码496个氨基酸,与斑马鱼Slc7a11基因氨基酸同源性达93.7%;鲫Pnp4a全长1535bp,编码291氨基酸,与斑马鱼Pnp4a基因氨基酸同源性达95.1%。RT-PCR检测了Mitfa、Tyr、Slc7a11和Pnp4a在白鲫、红鲫、花鲫三种不同体色鲫胚胎及成体组织中的表达,分析结果显示:花鲫和红鲫一样有一个黑色素消退过程,鲫Slc7a11和Pnp4a体色基因与斑马鱼Slc7a11和Pnp4a具有高度同源性,花鲫成体皮肤组织也和红鲫成体皮肤组织一样检测到了Mitfa和Tyr的表达。Pnp4a在不同鲫的不同体色发育时期和不同组织都有表达,Slc7a11在不同鲫的不同体色发育时期均有表达,但在不同鲫的成体皮肤组织中均未表达。
The early body color formation of red-white crucian carp was observed. Melanocytes firstly appear before fertilization, then xanthophores happen and later iridophore appear. And then, the body colour of red-white crucian carp changes from dark grey to red- orange after about 3 months old. the full-length cDNAs for Slc7a11 and Pnp4a were isolated. Our results revealed that the full-length Pnp4a cDNA contains 1 535 bp, with an open reading frame of 783 nucleotides, which encodes a deduced protein of 291 amino acids, coding for a deduced sequence alignment showed that crucian carp Pnp4a shares high levels of identity with those from Danio rerio (95.1%). The full-length Slc7a11 cDNA contains 1 782 bp, with an open reading frame of 1488 nucleotides, which encodes a deduced protein of 496 amino acids. The amino acid sequence alignment demonstrated that the red crucian carp Slc7a11 protein shared a sequence identity of 93.7% with Danio rerio, Oryzias latipes .The expression of Mitfa , Tyr , Slc7a11 and Pnp4a in three kinds of different color crucian carp embryos and adult tissues were detected by RT-PCR. The results showed that the red-white crucian carp has the same melanin decay process as the red crucian carp.Amino acid sequence alignment showed that Pnp4a and Slc7a11 of red crucian carp share high identity with those from Cyprinus carpio .The adult skin tissue of the red-white crucian carp was detected the expression of Mitfa and Tyr ,so did the adult skin tissue of the red crucian carp. Pnp4a was expressed in different body color development stages and different tissues of different crucian carp. Slc7a11 was expressed in different body color development stages of different crucian carp. However,it was not detected in different adult skin tissues of different crucian carp.
作者
范云鹏
赵韩
罗世民
唐玲倩
彭亮跃
刘文彬
肖亚梅
刘锦辉
FAN Yunpeng;ZHAO Han;LUO Shiming;TANG Lingqian;PENG Liangyue;LIU Wenbin;XIAO Yamei;LIU Jinhui(State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changshang 410081, China;Hunan Vocational College of Science and Technology, Changshang 410081, China)
出处
《激光生物学报》
CAS
2019年第4期343-352,373,共11页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(31772902,31802284,31472272)
湖南省自然科学基金项目(2018JJ3345)
湖南省科技计划重点研发计划资助项目(2016NK2046,2017SK2302)
长沙市科技计划项目(kq1701046)
