摘要
建立了一种同时测定水中甲萘威和阿特拉津含量的高效液相色谱分析法。样品经乙酸乙酯萃取净化后,使用XDB-C18色谱柱和紫外检测器,以甲醇∶水=60∶40(V/V)为流动相,流速1.0mL/min,柱温25℃,进样量10.0μL,在221nm波长下进行测定。结果表明,在0.03~1.00mg/L甲萘威和阿特拉津的峰面积与其质量浓度间均呈良好的线性关系,相关系数均为0.9999,最低检出限分别为0.089μg/L和0.184μg/L。在添加水平为0.2~1.0μg/L水样中甲萘威和阿特拉津的平均回收率分别为85.12%~100.14%和82.67%~105.51%,相对标准偏差分别为1.60%~4.69%和2.36%~4.26%。该方法简单、快速、准确、灵敏,具有良好的精密度和准确度,适用于常规检测分析。
A method for simultaneous analysis of carbaryl and atrazine content in water was established by high performance liquid chromatography(HPLC) with ultraviolet detector. Samples were extracted and purified by ethyl acetate. The XDB-C18 column was used as chromatography column, and the mobile phase was a mixture of methanol-water(60∶40,V/V) at a flow rate of 1.0 mL/min. The column temperature kept 25 ℃, injection volume was 10.0 μL and the wavelength of detector was 221 nm. The results showed that within the range of 0.03~1.00 mg/L, it had good linear relationship between the peak area of carbaryl and atrazine and its mass concentration, and the linear correlation coefficient for carbaryl and atrazine was 0.999 9 and 0.999 9 respectively. The minimum detecion limits of quantitation of carbaryl and atrazine was 0.089 μg/L and 0.184 μg/L respectively. On addition level of 0.2~1.0 μg/L range, the recovery of carbaryl and atrazine for water was 85.12%~100.14% and 82.67%~105.51% respectively, and relative standard deviation(RSD) respectively was 1.60%~4.69% and 2.36%~4.26%. The method could be used for conventional analysis, which was simple, rapid, sensitive, accurate and with good precision.
作者
赵文晋
顾桂飞
ZHAO Wen-jin;GU Gui-fei(Environmental Monitoring Station of Bijie,Bijie 551700,Guizhou,China;Zhouyi Tea Plantation,Bijie 551700,Guizhou,China)
出处
《湖北农业科学》
2019年第15期111-114,共4页
Hubei Agricultural Sciences
基金
贵州省科技支撑计划项目(黔科合支撑[2016]2534号)
黔科合重大专项([2018]3013)
贵州省科学技术基金计划项目(黔科合基础[2017]1083)
关键词
甲萘威
阿特拉津
紫外检测
HPLC
carbaryl
atrazine
ultraviolet detection
high performance liquid chromatography
