摘要
目的研究不同环境条件对脓毒症相关性脑病(sepsis-associated encephalopathy, SAE)认知功能的影响和机制。方法成年雄性C57BL/6小鼠96只,3~4月龄。采用腹腔注射脂多糖(lipopolysaccharide,LPS)建立脓毒症相关性脑病模型,分为生理盐水+标准环境(SE)组(NS组)、生理盐水+丰富环境(EE)组(NE组)、生理盐水+恶劣环境(IE)组(NI组)、LPS+SE组(LS组)、LPS+EE组(LE组)和LPS+IE组(LI组)。应用矿场实验检测小鼠运动总距离和中央格停留时间,应用条件恐惧实验检测小鼠场景性僵直时间和条件性僵直时间。检测前额叶皮质和海马炎性因子(TNF-α,IL-1β,IL-6,IL-10)浓度,脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)含量。结果 NI组和LS组场景性僵直时间明显短于NS组(P<0.05);LE组场景性僵直时间明显长于LS组(P<0.05);LI组场景性僵直时间明显短于NI组、LS组和LE组(P<0.05)。NI组和LS组海马IL-6浓度明显高于NS组(P<0.05),海马BDNF含量明显低于NS组(P<0.05)。结论恶劣环境可增加脓毒症脑病海马炎性因子的水平,加重认知损伤,而丰富环境可降低炎性因子的水平,改善脓毒症脑病的认知损伤。
Objective To investigate the effects and mechanisms of different environmental conditions on cognitive function in mice with sepsis-associated encephalopathy(SAE). Methods Ninety-six male C57 BL/6 mice, aged 3-4 months, were established into a model of sepsis-associated encephalopathy by intraperitoneal injection of lipopolysaccharide(LPS). They were divided into saline + standard environment(SE) group(group NS), saline + rich environment(EE) group(group NE), saline + harsh environment(IE) group(group NI), LPS + SE group(group LS), LPS + EE group(group LE) and LPS + IE group(group LI). Total distance and time in center were measured by open field test. Freezing to contest and Freezing to cue were detected by fear conditioning test. The levels of inflammatory cytokines(TNF-α, IL-1β, IL-6, IL-10) in the prefrontal cortex and hippocampus and the content of brain derived neurotrophic factor(BDNF) were detected. Results The freezing time of NI and LS groups was significantly shorter than that of group NS(P < 0.05). The freezing time of group LE was significantly longer than that of group LS(P < 0.05). The freezing time of the group LI was significantly shorter than that of the group NI, group LS and group LE(P < 0.05). The concentration of IL-6 in hippocampus of group NI and group LS was significantly higher than that of group NS(P < 0.05). The content of BDNF in hippocampus was significantly lower than that in group NS(P < 0.05). Conclusion IE can increase the hippocampous inflammatory level in SAE, deteriorate the impairment of cognition, while EE can decrease the inflammatory level,improve the impairment of cognition induced by SAE.
作者
毛明杰
张玲
杨建军
周志强
MAO Mingjie;ZHANG Ling;YANG Jianjun;ZHOU Zhiqiang(Department of Anesthesiology ,Medical School of Nanjing University, Nanjing General Hospital, Nanjing 210002, China)
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2019年第7期693-696,共4页
Journal of Clinical Anesthesiology
基金
国家自然科学基金(81571083)
关键词
脓毒症
环境条件
炎症反应
认知功能
Sepsis
Environmental conditions
Inflammatory response
Cognitive function
