摘要
为了寻找高效纤维素降解菌,提高秸秆降解效果并缩短秸秆腐解时间,从腐烂秸秆及附近土壤中,筛选获得高效秸秆纤维素降解细菌,并研究其对秸秆纤维素的降解能力。利用羧甲基纤维素钠培养基分离纤维素降解菌,结合纤维素刚果红测定、滤纸条降解试验和秸秆失重法筛选到2株具有纤维素降解能力的细菌(CMC-red、CMC-I),经16S r DNA序列分析,初步鉴定菌株CMC-red为Massilia arvi菌属,菌株CMC-I为黄杆菌属(Flavobacterium banpakuense)。菌株CMC-red的降解能力强,10 d可将滤纸降解成糊状,10 d内对秸秆的降解率可达24. 14%。通过分析红外光谱和扫描电镜图可以得出,经纤维素降解菌降解的秸秆纤维素、半纤维素的吸收峰减弱,纤维素的结构变得疏松。筛选获得的2株细菌中,菌株CMC-red对秸秆具有显著的降解效果。
In order to find efficient cellulose-degrading bacteria,improve the degradation effect of stalk and shorten the decomposing time of stalk,the high-efficient cellulose-degrading bacteria were isolated from the decayed leaves and nearby soil and the degradation ability of straw cellulose was studied. With CMC-Na as sole carbon source,based on the results of Congo red cellulose hydrolytic hole measurement,filter paper degradation test and straw weight loss method,two strains with high cellulose degradation efficiencies were obtained. 16 S r DNA sequencing analysis showed that strain CMC-red was Massilia arvi and strain CMC-I was Flavobacterium banpakuense. Compared with CMC-I,strain CMC-red had high stalk degradation ability,which could degrade filter paper into paste within 10 days,and the weight loss rate of corn stalk after 10 days was 24. 14%. The absorption peaks of stalk cellulose and hemicellulose degraded by cellulose degrading bacteria were weakened by the infrared spectrum and scanning electron microscope. The structure of cellulose was destroyed.Thus,the results showed that the strain of CMC-red had higher stalk degradation effect.
作者
孙玲
吴景贵
李建明
范围
王彩云
姚颜莹
SUN Ling;WU Jinggui;LI Jianming;FAN Wei;WANG Caiyun;YAO Yanying(College of Resources and Erwironment, Jilin Agricultural University, Changchun 130118, China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2019年第4期402-407,共6页
Journal of Jilin Agricultural University
基金
吉林省重大科技招标专项(20150203004NY)
国家重点研发计划项目(2017YFD0201801)
