摘要
目的比较中国妊娠期糖尿病母亲与正常母亲母乳中N-聚糖的岩藻糖基化水平差异,分析这些差异对其子代肠道微生态的影响。方法收集妊娠期糖尿病母亲和健康母亲在哺乳期第6天和第42天母乳样本各15例,及其纯母乳喂养的新生儿在同一天的粪便样本;以凝集素AAL分析母乳中N-聚糖的岩藻糖基化水平;以变性梯度凝胶电泳(DGGE)检测两组新生儿肠道菌群差异。结果妊娠期糖尿病母亲母乳N-聚糖岩藻糖基化水平显著高于健康母亲(t=4.438,P<0.01;t=3.238,P<0.001);两组新生儿肠道菌群存在明显差异,其中主要的微生物群如长双歧杆菌属、肠球菌属、泛菌属等的相对丰度有所不同。结论母乳中N-聚糖岩藻糖基化水平在妊娠期糖尿病母亲母乳中显著升高,这一变化可能影响其子代的肠道菌群结构。
Objective To observe the difference in levels of fucosylated N-glycans between Chinese mothers with gestational diabetes mellitus(GDM)and healthy controls,and analyze the effects of these differences on the gut microbiota of their breastfed infants.Methods Milk samples from 15 cases mothers with gestational diabetes and healthy controls and stool samples of breast-fed newborns were collected on the 6 th and 42 nd day of lactation.The differences in the levels of fucosylated N-glycans in breast milk were analyzed by using lectin blot.The differences in neonatal gut microbiota between the two groups were detected using denatured gradient gel electrophoresis(DGGE).Results There was a significant difference in the levels of fucosylated N-glycans between the mothers with gestational diabetes mellitus and healthy mothers(t=4.438,P<0.01;t=3.238,P<0.001),and in the structure of intestinal microbiota of their newborns.Conclusion Compared with healthy mothers,the levels of fucosylated N-glycans in mothers with gestational diabetes mellitus is significantly higher,which may significantly affects the structures of the gut microbiota of their offsprings.
作者
周娇锐
范青杰
刘曼
刘赫
李健新
胡雨奇
张利龙
许尧
李文哲
袁杰力
李明
ZHOU Jiaorui;FAN Qingjie;LIU Man;LIU He;LI Jianxin;HU Yuqi;ZHANG Lilong;XU Yao;LI Wenzhe;YUAN Jieli;LI Ming(Dalian Medical University,Dalian,Liaoning 116044,China)
出处
《中国微生态学杂志》
CAS
CSCD
2019年第7期752-756,762,共6页
Chinese Journal of Microecology
基金
辽宁省教育厅科学研究一般项目(L2016262)
广州合生元营养护理研究院母婴营养与护理研究基金(BINCMCF1102)
关键词
妊娠期糖尿病
岩藻糖
新生儿
肠道菌群
Gestational diabetes mellitus
Fucose
Neonate
Gut microbiota
