摘要
目的:探讨助阳通便膏对大鼠便秘的肠道菌群及屏障功能的调节作用。方法:将60只昆明大鼠,雌雄各半,随机分成3组(正常组、模型组、助阳通便膏组),每组20只,除正常组外均与洛哌丁胺进行小鼠便秘模型造模。对比各组造模前、造模后、治疗后的肠道菌群变化。结果:与正常组相比,所有便秘组大肠杆菌科的数量相对增加,双歧杆菌属、乳酸杆菌属、梭状芽孢杆菌属和普雷西氏杆菌属的数量均有所减少。且助阳通便膏能够增加Zo-1的表达水平,降低Cldn-1的水平,有助于恢复受损的结肠屏障功能,调节水电解质渗透,从而增加粪便的排泄量。结论:助阳通便膏通过调节便秘大鼠的肠道菌群,维持肠道菌群稳态,并且能够修复结肠屏障,调节水电解质渗透,从而改善便秘症状。
Objective:To explore the regulation effect of Zhuyang Tongbian Ointment on intestinal flora and barrier function in rats with constipation. Methods: Sixty Kunming rats,half male and half female,were randomly divided into three groups( normal group,model group,and Zhuyang Tongbian Ointment group),20 rats in each group. The mice constipation model was set up with loperamide in addition to the normal group. The changes of intestinal flora before and after treatment were compared. Results: Compared with the normal group,the number of Escherichia coli families in all constipation groups increased relatively,and the number of bifidobacterium,lactobacillus,clostridium,and prysosporium decreased. And Zhuyang Tongbian Ointment can increase the expression level of Zo-1,reduce the level of Cldn-1,help to restore the damaged colon barrier function,regulate the water and electrolyte penetration,and increase the excretion of feces. Conclusion: Zhuyang Tongbian Ointment can maintain the intestinal flora homeostasis Iby regulating the intestinal flora of constipation rats,and can repair the colon barrier and regulate the penetration of water and electrolytes,and thus improving the symptoms of constipation.
作者
李海波
石宇
张虹玺
LI Haibo;SHI Yu;ZHANG Hongxi(Basic Medicine College,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Graduate School,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110003,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第7期1714-1717,共4页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省自然科学基金项目(20180550953)
关键词
助阳通便膏
洛哌丁胺
便秘
肠道菌群
Zhuyang Tongbian Ointment
loperamide
constipation
intestinal flora