摘要
目的观察采用富氢盐水干预对严重高电压烧伤大鼠肾功能及肾组织病理变化的影响。方法选取40只健康大鼠,其中30只制备严重高压电烧伤模型,建模后随机分为富氢盐水组、药物对照组和模型组,其余10只未进行高压电击,设为对照组。电击后立即分别对富氢盐水组给予富氢盐水,药物对照组给予乌司他丁,模型组和对照组给予生理盐水腹腔注射。对比各组电击后2、4 h血尿素氮(BUN)、血肌酐(Cr)水平;取肾组织进行苏木精-伊红(HE)染色观察各组肾组织病理学变化;采用脱氧核苷酸缺口原位末端标记(TUNEL)法检测并对比各组肾细胞凋亡情况;分别采用实时荧光定量聚合酶链反应法和蛋白免疫印迹法检测并对比各组肾组织中缺氧诱导因子(HIF)-1α、B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)mRNA和蛋白表达情况。结果血清BUN、Cr水平依对照组→富氢盐水组→药物对照组→模型组之序递升,组间总体比较及两两比较差异均有统计学意义(P<0.01,P<0.05);富氢盐水组、药物对照组干预4 h低于干预2 h(P均<0.05),对照组、模型组干预2 h和4 h比较差异无统计学意义(P均>0.05)。对照组大鼠肾组织无明显病理学变化,模型组肾小球体积增大、间质血管充血、肾小管上皮细胞空泡变性,管腔可见细胞及蛋白管型,药物对照组和富氢盐水肾组织病理变化均有所减轻,其中富氢盐水组减轻更为明显。肾细胞凋亡率、肾组织中HIF-1α、Bcl-2 mRNA和蛋白相对表达量依对照组→富氢盐水组→药物对照组→模型组之序递升,Bax mRNA和蛋白相对表达量依此序递降,组间总体比较及两两比较差异均有统计学意义(P<0.01,P<0.05)。结论富氢盐水可显著改善严重高电压烧伤大鼠肾功能,促进受损肾组织恢复,减少肾细胞凋亡,可能与其抑制HIF-1α、Bcl-2 mRNA和蛋白表达、促进Bax mRNA和蛋白表达有关。
Objective To observe the effects of hydrogen-rich saline on renal function and histopathological changes in rats with severe high-voltage electrical burn.Methods Out of 40 healthy rats,30 rats were randomly divided into hydrogen-rich saline group,drug control group and model group after modeling of high-voltage electrical shock burn.The remaining 10 rats were set as control group without electric shock.Immediately after the electric shock,intraperitoneal injections of hydrogen-rich saline and ulinastatin were given in hydrogen-rich saline group and drug control group respectively,and intraperitoneal injections of normal saline were given in model group and control group.At 2-and 4-hour after electric shock,serum urea nitrogen(BUN) and creatinine(Cr) levels were compared among the groups;renal tissue was taken for HE staining to observe the pathological changes;by TUNEL,renal cell apoptosis was detected and compared;using real-time fluorescence quantification polymerase chain reaction and Western blotting,hypoxia-inducible factor-1α(HIF-1α),B lymphocyte tumor-2 gene(Bcl-2),Bcl-2 related X protein(Bax) mRNA expressions and proteins were detected and compared.Results Serum BUN and Cr levels increased statistically in the order of control group,hydrogen-rich saline group,drug control group and model group,and there were significant differences in their intra-groups comparisons and pairwise comparisons(P<0.01,P<0.05).At 4 h after intervention,serum BUN and Cr levels in hydrogen-rich saline group and drug control group were significantly lower than those at 2 h after intervention(all P<0.05),but there were no significant differences in them at 2-and 4-hour after intervention between control group and model group(all P>0.05).No obvious pathological changes in renal tissue were found in control group.Glomerular enlargement,interstitial vascular congestion,vacuolar degeneration of renal tubular epithelial cells,and luminal visible cells and protein casts appeared in model group.The pathological changes of kidney tissue
作者
何小龙
张兵
李峥
李巍
HE Xiao-long;ZHANG Bing;LI Zheng;LI Wei(Department of Bum, Sichuan People's Hospital, Sichuan Academy of Medical Sciences, Chengdu, Sichuan 610000, China)
出处
《中国临床研究》
CAS
2019年第7期874-878,共5页
Chinese Journal of Clinical Research
