摘要
【目的】为两亲性大分子季铵盐对植物病原真菌的抑菌活性表征方法的选择提供参考。【方法】分别采用生长速率法和菌丝称重法表征一类新型两亲性大分子季铵盐〔聚二甲基硅氧烷-b-聚丙烯酸酯季铵盐嵌段共聚物,PDMS-b-(PDMAEMA-BC),简称Six-Q5〕、亲水性大分子季铵盐(PQD-BC)和小分子季铵盐(BC)对水稻纹枯病菌(Rhizoctonia solani)的抑菌活性。【结果】生长速率法可用于PQD-BC和BC的抑菌活性表征,但不适合Six-Q5的抑菌活性表征;菌丝称重法可用于3类季铵盐抑菌活性的表征。PDMS嵌段长度适中的Si5-Q5对R.solani的IC50与IC90分别为12、59μg/mL,其抑菌活性与小分子季铵盐相当。【结论】对于两亲性大分子季铵盐,菌丝称重法较生长速率法更适合其抑菌活性的表征。
【Objective】This study provided a reference for the selection of characterization methods of amphiphilic macromolecule quaternary ammonium salts(QAS)on plant pathogenic fungi.【Method】The antifungal activity of new amphipathic macromolecular QAS copolymers〔PDMS-b-(PDMAEMA-BC),labeled as Six-Q5〕,water soluble macromolecule QAS(PQD-BC)and small molecular QAS(benzalkonium chloride,BC)against Rhizoctonia solani were characterized by growth rate method and mycelium weighing method respectively.【Result】Growth rate method can be used to characterize the antimicrobial activity of hydrophilic macromolecular QAS(PQD-BC)and small molecular QAS(BC),but not used to characterize amphiphilic macromolecular QAS.Mycelial weighing method can be used to characterize the antimicrobial activity of three kinds of QAS.The IC50 and IC90 of Si5-Q5 with moderate length of PDMS block for R.solani were 12μg/mL and 59μg/mL respectively,and its antifungal activity was similar to that of small molecular QAS.【Conclusion】For amphipathic macromolecule QAS,mycelial weighing method was more suitable than growth rate method for the characterization of antifungal activity.
作者
董辰韵
常瑶瑶
钟伟强
张安强
林雅铃
DONG Chenyun;CHANG Yaoyao;ZHONG Weiqiang;ZHANG Anqiang;LIN Yaling(College of Materials and Energy,South China Agricultural University,Guangzhou 510642;School of Material Science and Engineering,South China University of Technology,Guangzhou 510641)
出处
《广东农业科学》
CAS
2019年第6期86-92,共7页
Guangdong Agricultural Sciences
基金
广州市科技计划项目(201704020084,201803020015)
国家自然科学基金(31772202)
关键词
两亲性大分子季铵盐
生长速率法
菌丝称重法
水稻纹枯病菌
amphipathic macromolecular quaternary ammonium salt
growth rate method
mycelium weighing method
Rhizoctonia solani
