摘要
利用黄曲霉毒素专用测定仪建立了一种快速检测食品中黄曲霉毒素B1的分析方法,该仪器采用基于LED光源的荧光检测器和光衍生器。试样采用乙腈提取,226多功能净化柱净化,净化液经C18反相色谱柱分离利用黄曲霉毒素衍生物的荧光特性进行含量测定。结果表明,样品中黄曲霉毒素B1在0.01~20μg/kg内线性关系良好,相关系数为0.9998,S/N3信噪比下检出限0.007μg/kg,实际样品的加标回收率在80%~90%之间,同一样品重复测定结果的相对标准偏差0.69%,方法检出限、精密度、准确性等指标均满足国家标准限量要求。
The aflatoxin-analyzer uses fluorescence detector and photochemical derivative based on LED light source.The samples extracted by acetonitrile,pretreated by 226 multi-functional purifying column,separated by C18 reversed-phase chromatography column,then aflatoxin was determined by fluorescence detector.Linearity of aflatoxin B1 in samples is between(0.01-20)ppb.The correlation coefficient is 0.9998,the detection limit under triple signal-to-noise ratio is 0.007 ppb,the recovery rate of practical samples is between 80%-90%,the relative standard deviation of repeated determination of the same sample is 0.69%.The method detection limit,precision,accuracy and other indicators all can meet the requirement of China national standard.
作者
张习志
邓立刚
秦宏伟
王雨心
赵杨兰
邵光印
赵玉华
赵桂茂
许爱华
Zhang Xizhi;Deng ligang;Qin Hongwei;Wang Yuxin;Zhao Yanglan;Shao Guangyin;Zhao Yuhua;Zhao Guimao;Xu Aihua(Institute of Quality Standard & Testing Technology for Agro-products, Jinan 250010,China;QingDao SHINE Environment Technology Co.,Ltd., Qingdao 266011,China;Shandong Institute of Metrology,Jinan 250014,China;Shandong University,Jinan 250014,China;National Test Institute of Metrology,Chengdu 610212,China)
出处
《分析仪器》
CAS
2019年第4期48-52,共5页
Analytical Instrumentation
基金
山东省重点研发计划“农产品及食品中黄曲霉毒素专用测定仪的开发及应用”(2017GSF20103)
关键词
荧光检测器
光衍生器
黄曲霉毒素
Fluorescence detector
Photochemical derivative
Aflatoxin