摘要
目的分析胸腔积液细胞蜡块免疫组化检测对胸腔积液的价值。方法方便选取2018年2月—2019年2月该院诊断的96例胸腔积液病患,进行随机分组,46例病患进行单纯细胞涂片HE染色作为参照组,50例病患进行细胞蜡块免疫组化检测作为探究组,观察并对比诊断结果。结果不同方式诊断后对比两组诊断准确率,探究组98.00%显著高于参照组86.96%(χ^2=4.32,P<0.05);对比两组检测结果的阳性率,探究组阳性率100.00%显著高于参照组78.26%(χ^2=10.45,P<0.05);96例胸腔积液病患中,48例诊断为查见腺癌细胞,48例为查见反应性增生间皮细胞。从抗体表达来看,发现腺癌细胞的特异性抗体有CK7、CEA和TTF-1,反应性增生间皮细胞的特异性抗体有WT-1、Calretinin和CK5/6(P<0.05)。结论细胞蜡块免疫组化检测对胸腔积液的诊断准确率高,值得临床推广。
Objective To analyze the value of immunohistochemical detection of pleural effusion cells in pleural effusion. Methods A total of 96 patients with pleural effusion diagnosed in our hospital from February 20 to February 2019 were convenient enrolled in a randomized group. 46 patients underwent HE staining with a single cell smear as a reference group, and 50 patients underwent cell waxing block immunohistochemistry was used as an exploration group to observe and compare the diagnosis results. Results After diagnosis, the diagnostic accuracy of the two groups was compared. 98.00% of the exploration group was significantly higher than the reference group by 86.96%(χ^2=4.32, P<0.05). The positive rate of the two groups was 100.00%, higher than the reference group of 78.26%(χ^2=10.45, P<0.05);of the 96 patients with pleural effusion, 48 were diagnosed as adenocarcinoma cells, and 48 were found to be reactive hyperplastic mesothelial cells. From the expression of antibodies, specific antibodies against adenocarcinoma cells were found to be CK7, CEA and TTF-1, and specific antibodies to reactive proliferative mesothelial cells were WT-1, Calretinin and CK5/6 (P<0.05). Conclusion The immunohistochemical detection of cell wax block has a high diagnostic accuracy for pleural effusion, which is worthy of clinical promotion.
作者
姜洪翠
JIANG Hong-cui(Department of Pathology, the First Hospital of Zibo, Zibo, Shandong Province, 255000 China)
出处
《中外医疗》
2019年第17期23-25,共3页
China & Foreign Medical Treatment
关键词
胸腔积液
细胞蜡块免疫组化检测
Pleural effusion
Immunohistochemistry detection of cell wax block
