摘要
目的观察姜黄素衍生物(FM0807)的镇痛作用。方法(1)末次给药90min后,每只小鼠腹腔注射0. 6%醋酸溶液0. 2 m L构建化学刺激疼痛模型。按照体重将小鼠随机分为5组:模型组、对照组和低、中、高3个剂量实验组,每组8只。模型组灌胃给予0. 9%Na Cl,对照组灌胃给予阿司匹林74 mg·kg^-1,低、中、高3个剂量实验组分别给予FM0807:50,100,200 mg·kg^-1,连续给药5 d。记录腹腔注射醋酸溶液后15 min内的小鼠扭体次数并计算扭体抑制率。(2)末次给药90 min后,于大鼠右后爪的跖面皮内注射角叉菜胶建立炎症疼痛模型。按体重将大鼠随机分为5组:模型组、对照组和低、中、高3个剂量实验组,每组8只。模型组灌胃给予0. 9%Na Cl,对照组灌胃给予阿司匹林37 mg·kg^-1,低、中、高3个剂量实验组分别给予FM0807:25,50,100 mg·kg^-1,连续给药5 d。用机械缩足反射痛阈值测定法测造模前和造模后1,3和6 h的痛阈值。结果(1)小鼠化学刺激疼痛:模型组、对照组和低、中、高3个剂量实验组的扭体抑制率分别为0%,50. 95%,25. 10%,45. 25%和69. 20%;对照组和中、高剂量2个实验组与模型组相比,差异均有统计学意义(P <0. 05,P <0. 001)。(2)注射角叉菜胶大鼠炎症疼痛:模型组、对照组和低、中、高3个剂量实验组6 h的痛阈值分别为(22. 11±4. 16),(34. 28±2. 37),(26. 34±5. 24),(31. 78±2. 85)和(36. 55±2. 62) g,对照组和中、高剂量2个实验组与模型组相比,差异均有统计学意义(均P <0. 001)。结论 FM0807对醋酸和角叉菜胶诱发的外周疼痛具有明显的镇痛作用。
Objective To observe analgesic effect of curcumin derivative FM0807. Methods ( 1) Mice were intraperitoneally injected with 0. 6 % acetic acid solution,0. 2 mL/mouse,90 min after the final administration,and a chemical stimulation pain model was established. The mice were randomly divided into 5 groups: model group,control group, and low,medium and high dose experimental groups,8 in each group. The model group was given normal saline by gavage;control group was given aspirin 74 mg·kg^-1 by gavage;the low,medium and high dose experimental groups were given FM0807: 50,100,200 mg·kg^-1 for 5 consecutive days. The times of torsion of mice within 15 min after intraperitoneally injected with acetic acid solution were recorded and the inhibition rate of torsion was calculated.( 2) After the final administration for 90 min,the rat model of inflammation and pain was established by intradermal injection of carrera on the metatarsal surface of the right hind paw. Rats were randomly divided into 5 groups: model group,control group,and low,medium and high dose experimental groups,8 in each group. The model group was given normal saline by gavage;control group was given aspirin 37 mg·kg^-1 by gavage;the low,medium and high dose experimental groups were given FM0807: 25,50,100 mg·kg^-1 for 5 consecutive days. The pain thresholds of pre - administration and 1 h, 3 h and 6 h after modeling were measured by the mechanical pedometer reflex method. Results ( 1) In the acetic acid - induced mouse writhing test: the torsion inhibition rates of model group,control group and low,medium and high dose experimental groups were respectively 0. 0 %,50. 95 %,25. 10 %,45. 2 5% and 69. 20 %. Comparison between the control group and the medium and high dose experimental groups,the differences were statistically significantly ( P < 0. 05,P < 0. 001).( 2) In the carrageenan - induced inflammation pain experiment of rats: the pain thresholds at 6 h in the above 5 groups were ( 22. 11 ± 4. 16) g,( 34. 28 ± 2. 37) g,( 26. 34 ± 5. 24) g,( 31. 7
作者
罗玉蓉
吴伟芳
许钊溢
杨泽梁
魏慧泉
范芸
林玉滢
谢廷良
张南文
LUO Yu-rong;WU Wei-fang;XU Zhao-yi;YANG Ze-liang;WEI Hui-quan;FAN Yun;LIN Yu-ying;XIE Ting-liang;ZHANG Nan-wen(School of Clinic Medical,Fujian Medical University, Fuzhou 350122,Fujian Province,China;School of Pharmacy,Fujian Medical University, Fuzhou 350122,Fujian Province,China;Department of Anesthesiology,Fuzhou Children’s Hospital of Fujian Province, Fuzhou 350005,Fujian Province,China;Department of Pharmacology,Fuqing Health School of Fujian Province,Fuqing 350313,Fujian Province,China;Fujian Major Laboratory of Natural Medicine Pharmacology,Fuzhou 350122,Fujian Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2019年第13期1346-1348,共3页
The Chinese Journal of Clinical Pharmacology
基金
福建省卫生计生中青年骨干人才培养基金资助项目(2018-ZQN-61)
福建医科大学大学生创新创业训练计划基金资助项目(C18009)
福建省科技创新联合基金资助项目(2017Y9120)
关键词
姜黄素衍生物
镇痛
醋酸
角叉菜胶
痛阈
curcumin derivative
analgesia
acetic acid
carrageenan
pain threshold
