摘要
为探究microRNA-155(miR-155)对小鼠棕色脂肪组织分化的影响,收集广泛过表达miR-155的转基因小鼠(RL-m155小鼠)棕色脂肪组织,离体棕色脂肪大体拍照,然后收集部分组织固定、石蜡包埋和切片,并行HE染色,观察棕色脂肪细胞的大小和形态;同时RT-qPCR检测棕色脂肪组织中分化相关基因的表达水平。结果表明,RL-m155小鼠的体重、体型与对照小鼠无显著差异;与对照小鼠相比,RL-m155小鼠棕色脂肪组织中miR-155表达水平显著升高,而棕色脂肪组织体积及棕色脂肪细胞的大小均显著减小;棕色脂肪中特异表达基因(UCP1和SCA-1)、棕色脂肪分化调控因子(BMP2、BMP6、Smad1、Smad5、C/EBPB、MYO10和PTEN)、棕色脂肪分化诱导因子(PPARγ、PGC-1α、BMP2、BMP4、BMP7、BMPR1A和Sirt1)等棕色脂肪分化相关功能基因的mRNA表达水平均显著降低(P<0.05)。表明RL-m155小鼠棕色脂肪组织中miR-155过表达,导致棕色脂肪组织分化受损。
To explore the effects of miR-155 on the differentiation of brown adipose tissue in mice,brown adipose tissue (BAT) of miR-155 transgenic mice (i.e.,RL-m155 mice) and control mice were collected,fixed in 40 mg/mL paraformaldehyde overnight and embedded in paraffin,followed by hematoxylin and eosin staining (H&E staining).RT-qPCR was used to determine the expression of the indicated mRNA transcripts in BAT.These data demonstrated that RL-m155 mice displayed normal body weight,and reduced BAT weight and size.The size and number of lipid droplets, and the expression of key adipogenic and thermogenic factor, including brown fat specific expression genes (UCP1 and SCA-1),brown fat differentiation regulatory factors (BMP2,BMP6,Smad1,Smad5,CEBP/B,MYO10 and PTEN) and brown fat differentiation inducible factors (PPARγ,PGC-1α,BMP2,BMP4,BMP7,BMPR1A,Sirt1,etc.),in BAT of RL-m155 mice were clearly reduced.Our results indicated that miR-155 overexpression in BAT of RL-m155 mice impaires brown fat cell differentiation.
作者
黎海燕
刘宇
廉梅
陈恒伟
温悦婷
贾俊双
林晓琳
肖东
LI Hai-yan;LIU Yu;LIAN Mei;CHEN Heng-wei;WEN Yue-ting;JIA Jun-shuang;LIN Xiao-lin;XIAO Dong(Laboratory Animal Center,Southern Medical University,Guangzhou,Guangdong,510515,China;Cancer Research Institute,Southern Medical University,Guangzhou,Guangdong,510515,China)
出处
《动物医学进展》
北大核心
2019年第8期49-54,共6页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(81172587,81702778,81600488)
中国博士后科学基金项目(2017M622740,2018T110884)
